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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395083
gRNA Name: LOC588806 gRNA7
Synonyms: Pks-sgRNA4
Genomic Target: LOC588806
Derived from:

Sequence: 5' GGTATTGCGCACAGTGTGT 3'



Genomic Target S. purpuratus
Identities 19/19
genome
gRNA
 
5' ACACACTGTGCGCAATACC 3'
   |||||||||||||||||||
3' TGTGTGACACGCGTTATGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716109..2716127 19/19 Sense target LOC588806
NW_022145532.1:911197..911215 16/19 Antisense off-target LOC115923015
NW_022145612.1:33164745..33164763 16/19 Antisense off-target LOC100892147
NW_022145594.1:4919028..4919046 16/19 Antisense unknown
P. miniata 3.0 NW_024037055.1:14880982..14881000 17/19 Sense off-target LOC119723937
NW_024037072.1:5041821..5041839 16/19 Sense off-target LOC119740658
NW_024037059.1:41422387..41422405 15/19 Sense off-target LOC119729257
NW_024037048.1:3828463..3828481 15/19 Sense unknown
NW_024037048.1:4700850..4700868 15/19 Sense unknown
L. variegatus 3.0 chr9:26848954..26848972 16/19 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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