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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395082
gRNA Name: LOC588806 gRNA6
Synonyms: Pks-sgRNA3
Genomic Target: LOC588806
Derived from:

Sequence: 5' GTGGTGTCTTTGTCGGTAT 3'



Genomic Target S. purpuratus
Identities 19/19
genome
gRNA
 
5' ATACCGACAAAGACACCAC 3'
   |||||||||||||||||||
3' TATGGCTGTTTCTGTGGTG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716026..2716044 19/19 Sense target LOC588806
NW_022145596.1:25111416..25111434 15/19 Antisense off-target LOC585290
NW_022145596.1:21085081..21085099 15/19 Sense off-target LOC115918502
NW_022145596.1:13579660..13579678 16/19 Sense unknown
NW_022145599.1:10018380..10018398 16/19 Sense unknown
NW_022145599.1:9664913..9664931 16/19 Sense unknown
NW_022145609.1:39484309..39484327 15/19 Sense unknown
P. miniata 3.0 NW_024037076.1:10800297..10800315 15/19 Sense unknown
L. variegatus 3.0 chr15:27084738..27084756 18/19 Sense off-target LOC121428528
chr17:23790444..23790462 16/19 Sense off-target LOC121430457
chr14:19800218..19800236 15/19 Sense off-target LOC121427424
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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