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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395080
gRNA Name: LOC588806 gRNA4
Synonyms: Pks-sgRNA1
Genomic Target: LOC588806
Derived from:

Sequence: 5' GGGAGGTTCTCAAGGAAGGTA 3'



Genomic Target S. purpuratus
Identities 20/21
genome
gRNA
 
5' TACCTTCCTTGAGAACCTTCC 3'
   |||||||||||||||||| ||
3' ATGGAAGGAACTCTTGGAGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2715750..2715770 20/21 Sense target LOC588806
NW_022145560.1:1052299..1052319 17/21 Antisense off-target LOC588136
NW_022145606.1:47297931..47297951 17/21 Antisense off-target LOC752556
NW_022145571.1:880750..880770 17/21 Sense off-target LOC105444211
NW_022145605.1:41699697..41699717 17/21 Antisense off-target LOC100890682
NW_022144817.1:6233..6253 18/21 Antisense unknown
NW_022144817.1:6457..6477 18/21 Antisense unknown
NW_022145596.1:17412084..17412104 18/21 Sense unknown
NW_022145605.1:41695504..41695524 17/21 Antisense unknown
NW_022145605.1:41705872..41705892 17/21 Antisense unknown
NW_022145615.1:25327353..25327373 17/21 Antisense unknown
P. miniata 3.0 NW_024037059.1:30864512..30864532 18/21 Antisense off-target LOC119728608
NW_024037059.1:30864738..30864758 18/21 Antisense off-target LOC119728608
NW_024037062.1:5280082..5280102 18/21 Sense off-target LOC119731231
NW_024037062.1:5280308..5280328 18/21 Sense off-target LOC119731231
NW_024037062.1:5288731..5288751 18/21 Sense off-target LOC119731231
NW_024037062.1:5288957..5288977 18/21 Sense off-target LOC119731231
NW_024037065.1:1674645..1674665 18/21 Sense off-target LOC119733706
NW_024037069.1:33573365..33573385 18/21 Sense off-target LOC119738132
NW_024037047.1:20709684..20709704 18/21 Sense off-target LOC119745527
NW_024037059.1:30857765..30857785 18/21 Antisense off-target LOC119728608
NW_024037059.1:30857991..30858011 18/21 Antisense off-target LOC119728608
NW_024037059.1:6304122..6304142 17/21 Sense off-target LOC119727408
NW_024037059.1:30876756..30876776 18/21 Sense unknown
NW_024037064.1:14627273..14627293 18/21 Sense unknown
NW_024037065.1:2789224..2789244 18/21 Sense unknown
NW_024037069.1:27432032..27432052 18/21 Antisense unknown
NW_024037069.1:27432257..27432277 18/21 Antisense unknown
NW_024037069.1:27432482..27432502 18/21 Antisense unknown
NW_024037047.1:14708705..14708725 18/21 Sense unknown
NW_024037074.1:25040971..25040991 18/21 Sense unknown
NW_024037074.1:25040745..25040765 18/21 Sense unknown
NW_024037052.1:4601276..4601296 18/21 Antisense unknown
NW_024037052.1:4607364..4607384 18/21 Antisense unknown
NW_024037052.1:4607591..4607611 18/21 Antisense unknown
NW_024037058.1:11527749..11527769 18/21 Sense unknown
NW_024037058.1:11527975..11527995 18/21 Sense unknown
NW_024037058.1:11530363..11530383 18/21 Sense unknown
NW_024037048.1:2555375..2555395 17/21 Sense unknown
L. variegatus 3.0 chr10:1527068..1527088 18/21 Antisense off-target LOC121422560
chr5:37177615..37177635 18/21 Antisense off-target LOC121416060
chr5:40942711..40942731 18/21 Antisense off-target LOC121416163
Publications
???morpholino.displaySummary.papersFirst??? Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo., Dev Dyn 2017  
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