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Echinobase

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ECB-GRNA-26395079

gRNA Name: dll1 gRNA2
Synonyms: Delta-2 (20D11)
Genomic Target: dll1
Derived from:

Sequence: 5' GGCTACACGTGCCTCTGTCC 3'



Genomic Target S. purpuratus
Identities 20/20
genome
gRNA
 
5' GGACAGAGGCACGTGTAGCC 3'
   ||||||||||||||||||||
3' CCTGTCTCCGTGCACATCGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145611.1:35196036..35196055 20/20 Sense target dll1
NW_022145517.1:7217..7236 20/20 Antisense off-target LOC115921237
NW_022145613.1:13462122..13462141 19/20 Sense off-target LOC593680
NW_022145543.1:1244698..1244717 19/20 Antisense unknown
NW_022145599.1:24472665..24472684 19/20 Sense unknown
NW_022145543.1:1206267..1206286 18/20 Antisense unknown
NW_022145599.1:24497707..24497726 18/20 Sense unknown
NW_022145598.1:21917124..21917143 17/20 Sense unknown
NW_022145605.1:33430202..33430221 16/20 Sense unknown
P. miniata 3.0 NW_024037060.1:7750295..7750314 18/20 Antisense off-target LOC119729872
NW_024037060.1:7750409..7750428 18/20 Antisense off-target LOC119729872
NW_024037060.1:7750637..7750656 18/20 Antisense off-target LOC119729872
NW_024037060.1:7750754..7750773 18/20 Antisense off-target LOC119729872
NW_024037060.1:7791407..7791426 18/20 Antisense off-target LOC119729792
NW_024037066.1:3724955..3724974 18/20 Sense off-target LOC119734505
NW_024037076.1:8327527..8327546 18/20 Antisense off-target LOC119720398
NW_024037076.1:8327752..8327771 18/20 Antisense off-target LOC119720398
NW_024037076.1:8327866..8327885 18/20 Antisense off-target LOC119720398
NW_024037076.1:8328091..8328110 18/20 Antisense off-target LOC119720398
NW_024037076.1:8405191..8405210 18/20 Antisense off-target LOC119720728
L. variegatus 3.0 chr5:47453230..47453249 16/20 Antisense off-target LOC121416325
chr10:17827104..17827123 18/20 Antisense unknown
Publications
First: Notch signaling patterns neurogenic ectoderm and regulates the asymmetric division of neural progenitors in sea urchin embryos., Development 2017  
Most recent:
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