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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395076
gRNA Name: LOC580458 gRNA3
Synonyms: Spmesh 450
Genomic Target: LOC580458
Derived from:

Sequence: 5' GGCTAAAGTCGACATTGCTC 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' GAGCAATGTCGACTTTAGCG 3'
   ||||||||||||||||||| 
3' CTCGTTACAGCTGAAATCGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145594.1:28423738..28423757 19/20 Antisense target LOC580458
NW_022145594.1:28279382..28279401 18/20 Sense off-target LOC589327
NW_022145594.1:28385902..28385921 18/20 Sense off-target LOC586294
NW_022145615.1:32754741..32754760 17/20 Antisense off-target mef2cl
NW_022145544.1:1839518..1839537 17/20 Sense off-target LOC755676
NW_022145612.1:44270441..44270460 17/20 Antisense off-target LOC115919281
P. miniata 3.0 NW_024037057.1:7905638..7905657 16/20 Sense unknown
L. variegatus 3.0 chr1:10176425..10176444 18/20 Sense off-target LOC121425588
chr12:7504605..7504624 16/20 Antisense off-target LOC121425559
chr13:29494863..29494882 16/20 Antisense off-target LOC121426423
Publications
???morpholino.displaySummary.papersFirst??? Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
???morpholino.displaySummary.papersRecent??? Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2023  
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