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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395069
gRNA Name: gcml gRNA1
Synonyms: Sp.GCM.63(+)
Genomic Target: gcml
Derived from:

Sequence: 5' GCCGCCGGAGCTGCCGGGT 3'



Genomic Target S. purpuratus L. variegatus
Identities 19/19 18/19
genome
gRNA
 
5' ACCCGGCAGCTCCGGCGGC 3'
   |||||||||||||||||||
3' TGGGCCGTCGAGGCCGCCG 5'
 
5' ACCCGGTAGCTCCGGCGGC 3'
   |||||| ||||||||||||
3' TGGGCCGTCGAGGCCGCCG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145598.1:34773821..34773839 19/19 Sense target gcml
P. miniata 3.0 NW_024037056.1:13876646..13876664 16/19 Antisense off-target LOC119724727
NW_024037056.1:13876646..13876664 16/19 Antisense off-target LOC119724728
L. variegatus 3.0 chr17:1580502..1580520 18/19 Sense target gcml
chr1:54455455..54455473 17/19 Sense off-target zbtb8os
Publications
???morpholino.displaySummary.papersFirst??? Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo., Mol Reprod Dev 2016  
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