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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395068
gRNA Name: foxy gRNA3
Synonyms: SpFoxY 224
Genomic Target: foxy
Derived from:

Sequence: 5' GGCAGCCTTTGTAGGATGTT 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' AACATCCTACAAAGGCTGGC 3'
   |||||||||||||||||| |
3' TTGTAGGATGTTTCCGACGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145615.1:4516709..4516728 19/20 Antisense target foxy
NW_022145570.1:610556..610575 19/20 Sense off-target LOC575057
NW_022145599.1:31416681..31416700 19/20 Sense off-target LOC115918603
NW_022145615.1:3766304..3766323 19/20 Antisense off-target LOC115920611
NW_022145601.1:26341204..26341223 18/20 Antisense off-target LOC115918697
NW_022145610.1:24509344..24509363 16/20 Antisense off-target LOC588537
NW_022145610.1:24524976..24524995 16/20 Antisense off-target LOC115929149
NW_022145600.1:11627867..11627886 17/20 Antisense unknown
NW_022145603.1:19416091..19416110 17/20 Antisense unknown
NW_022145558.1:919443..919462 17/20 Antisense unknown
NW_022145613.1:32059585..32059604 16/20 Antisense unknown
P. miniata 3.0 NW_024037059.1:18454028..18454047 18/20 Antisense off-target tgm1
NW_024037065.1:6178205..6178224 18/20 Sense off-target mycbp2
NW_024037059.1:18454028..18454047 18/20 Antisense off-target LOC119727709
NW_024037071.1:6502859..6502878 18/20 Antisense off-target LOC119739847
NW_024037071.1:7348754..7348773 17/20 Sense off-target LOC119739687
NW_024037059.1:35304540..35304559 16/20 Antisense off-target uckl1
NW_024037073.1:4213179..4213198 16/20 Antisense off-target wdr20
NW_024037058.1:5822740..5822759 18/20 Antisense unknown
NW_024037048.1:9770745..9770764 16/20 Sense unknown
NW_024037069.1:5480597..5480616 16/20 Antisense unknown
L. variegatus 3.0 chr4:39644665..39644684 18/20 Antisense unknown
chr18:23275441..23275460 17/20 Antisense unknown
chr19:17869496..17869515 17/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
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