Summary Attributions Wiki

Genomic Target	S. purpuratus	A. planci		P. miniata		L. variegatus
Identities	19/20
genomic gRNA	5' AACATCCTACAAAGGCTGGC 3' |||||||||||||||||| | 3' TTGTAGGATGTTTCCGACGG 5'
Genomic Alignments	Position	Identity	Strand	Target	Genomic Target
	View All (0)
S. purpuratus 5.0	NW_022145615.1:4516709..4516728	19/20	Antisense	target	foxy
	NW_022145570.1:610556..610575	19/20	Sense	off-target	LOC575057
	NW_022145610.1:24509344..24509363	16/20	Antisense	off-target	LOC588537
	NW_022145599.1:31416681..31416700	19/20	Sense	off-target	LOC115918603
	NW_022145615.1:3766304..3766323	19/20	Antisense	off-target	LOC115920611
	NW_022145601.1:26341204..26341223	18/20	Sense	off-target	LOC115918697
	NW_022145610.1:24524976..24524995	16/20	Antisense	off-target	LOC115929149
	NW_022145558.1:919443..919462	17/20		unknown
	NW_022145600.1:11627867..11627886	17/20		unknown
	NW_022145603.1:19416091..19416110	17/20		unknown
	NW_022145613.1:32059585..32059604	16/20		unknown
P. miniata 3.0	NW_024037059.1:18454028..18454047	18/20	Sense	off-target	tgm1
	NW_024037059.1:35304540..35304559	16/20	Antisense	off-target	uckl1
	NW_024037073.1:4213179..4213198	16/20	Antisense	off-target	wdr20
	NW_024037065.1:6178205..6178224	18/20	Antisense	off-target	mycbp2
	NW_024037059.1:18454028..18454047	18/20	Antisense	off-target	LOC119727709
	NW_024037071.1:6502859..6502878	18/20	Sense	off-target	LOC119739847
	NW_024037071.1:7348754..7348773	17/20	Antisense	off-target	LOC119739687
	NW_024037058.1:5822740..5822759	18/20		unknown
	NW_024037048.1:9770745..9770764	16/20		unknown
	NW_024037069.1:5480597..5480616	16/20		unknown
L. variegatus 3.0	chr4:39644665..39644684	18/20		unknown
	chr18:23275441..23275460	17/20		unknown
	chr19:17869496..17869515	17/20		unknown
	View All (24)
Publications
First:	Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022
Most recent:
	View All Papers

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