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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395064
gRNA Name: LOC588806 gRNA2
Synonyms: Sp.PKS1.547(-)
Genomic Target: LOC588806
Derived from:

Sequence: 5' TGAGGGGTTTGAGGACAA 3'



Genomic Target S. purpuratus
Identities 18/18
genome
gRNA
 
5' TTGTCCTCAAACCCCTCA 3'
   ||||||||||||||||||
3' AACAGGAGTTTGGGGAGT 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145610.1:2716401..2716418 18/18 Antisense target LOC588806
NW_022145612.1:15987739..15987756 17/18 Antisense off-target LOC105439527
P. miniata 3.0 NW_024037058.1:2801087..2801104 17/18 Sense off-target drg1
XR_005271529.1:1193..1210 15/18 off-target LOC119732247
XR_005271530.1:538..555 15/18 off-target LOC119732247
XR_005271531.1:505..522 15/18 off-target LOC119732247
XR_005271532.1:355..372 15/18 off-target LOC119732247
XR_005271533.1:356..373 15/18 off-target LOC119732247
XR_005271534.1:394..411 15/18 off-target LOC119732247
XR_005271535.1:1147..1164 15/18 off-target LOC119732247
XR_005271536.1:914..931 15/18 off-target LOC119732247
XR_005271537.1:457..474 15/18 off-target LOC119732247
XR_005271538.1:311..328 15/18 off-target LOC119732247
XR_005271539.1:906..923 15/18 off-target LOC119732247
XR_005271540.1:323..340 15/18 off-target LOC119732247
XR_005271541.1:476..493 15/18 off-target LOC119732247
XR_005271542.1:457..474 15/18 off-target LOC119732247
XR_005271543.1:471..488 15/18 off-target LOC119732247
XR_005271544.1:309..326 15/18 off-target LOC119732247
XR_005271545.1:347..364 15/18 off-target LOC119732247
NW_024037053.1:568269..568286 15/18 Sense off-target LOC119722309
NW_024037052.1:12976597..12976614 16/18 Antisense unknown
L. variegatus 3.0 chr2:17980091..17980108 17/18 Antisense unknown
chr6:15915435..15915452 16/18 Antisense unknown
chr6:16890444..16890461 16/18 Antisense unknown
chr14:19620009..19620026 15/18 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo., Mol Reprod Dev 2016  
???morpholino.displaySummary.papersRecent??? CRISPR/Cas9 knockin methodology for the sea urchin embryo., Mol Reprod Dev 2023  
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