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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395062
gRNA Name: nanos2l gRNA4
Synonyms: SpNanos2 CKI1
Genomic Target: nanos2l
Derived from:

Sequence: 5' GGGTGTACGCCCTTAGTATC 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' GATACTAAGGGCGTACACCT 3'
   ||||||||||||||||||| 
3' CTATGATTCCCGCATGTGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145605.1:14906213..14906232 19/20 Sense target nanos2l
L. variegatus 3.0 chr2:69606340..69606359 18/20 Antisense off-target LOC121408828
Publications
???morpholino.displaySummary.papersFirst??? Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
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