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ECB-GRNA-26395057
gRNA Name:
nodall gRNA4
Synonyms: E2_777
Genomic Target: nodall
Derived from:
Sequence: 5' GGTAGGCGTTGAACTGCTTT 3'
Synonyms: E2_777
Genomic Target: nodall
Derived from:
Sequence: 5' GGTAGGCGTTGAACTGCTTT 3'
| Genomic Target | S. purpuratus | L. variegatus | |||||
| Identities | 20/20 | 20/20 | |||||
genome
gRNA
|
5' AAAGCAGTTCAACGCCTACC 3' |||||||||||||||||||| 3' TTTCGTCAAGTTGCGGATGG 5' |
5' AAAGCAGTTCAACGCCTACC 3' |||||||||||||||||||| 3' TTTCGTCAAGTTGCGGATGG 5' |
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Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | S. purpuratus 5.0 | NW_022145595.1:2654878..2654897 | 20/20 | Antisense | target | nodall |
| NW_022145600.1:26255920..26255939 | 18/20 | Antisense | off-target | LOC105442316 | |||
| P. miniata 3.0 | NW_024037061.1:15644621..15644640 | 18/20 | Sense | off-target | LOC119730627 | ||
| L. variegatus 3.0 | chr18:12696838..12696857 | 20/20 | Antisense | target | nodall | ||
| chr8:9818816..9818835 | 16/20 | Antisense | off-target | LOC121419950 | |||
| chr6:406069..406088 | 16/20 | Antisense | off-target | LOC121416721 | |||
| chr13:19161343..19161362 | 18/20 | Antisense | unknown | ||||
| Publications | |||||||
| ???morpholino.displaySummary.papersFirst??? |
Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016 |
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| ???morpholino.displaySummary.papersRecent??? | |||||||
| ???morpholino.displaySummary.papersAll??? | |||||||