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Echinobase

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ECB-GRNA-26395052
gRNA Name: abcb1 gRNA2
Synonyms: Sp-ABCB1a sgRNA E13-22
Genomic Target: abcb1
Derived from:

PAM Sequence: CGG
Sequence: 5' GAGCGAGGAGCTCAGCTGTC 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' GACAGCTGAGCTCCACGCTC 3'
   |||||||||||||| |||||
3' CTGTCGACTCGAGGAGCGAG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145604.1:6671918..6671937 19/20 Sense target abcb1
NW_022145601.1:21490568..21490587 18/20 Antisense off-target LOC582726
NW_022145601.1:27756481..27756500 18/20 Antisense off-target LOC587897
NW_022145601.1:27171562..27171581 18/20 Antisense off-target LOC115924608
NW_022145538.1:329976..329995 17/20 Antisense off-target LOC105439065
NW_022145538.1:342890..342909 17/20 Antisense off-target LOC115923431
NW_022145615.1:1281167..1281186 16/20 Sense off-target iqgap1
P. miniata 3.0 NW_024037055.1:7675925..7675944 17/20 Sense off-target LOC119723710
L. variegatus 3.0 chr14:10436011..10436030 18/20 Sense off-target LOC121427218
Publications
???morpholino.displaySummary.papersFirst??? CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae., J Exp Biol 2021  
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