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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395015
gRNA Name: nodall gRNA1
Synonyms: E1_157
Genomic Target: nodall
Derived from:

Sequence: 5' GGGCGTCCGGTGTGATAAG 3'



Genomic Target S. purpuratus
Identities 19/19
genome
gRNA
 
5' CTTATCACACCGGACGCCC 3'
   |||||||||||||||||||
3' GAATAGTGTGGCCTGCGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145595.1:2652607..2652625 19/19 Antisense target nodall
NW_022145599.1:10857151..10857169 15/19 Sense unknown
NW_022145599.1:11041593..11041611 15/19 Antisense unknown
P. miniata 3.0 NW_024037074.1:35314432..35314450 17/19 Sense off-target LOC119745886
NW_024037047.1:34505095..34505113 15/19 Sense off-target LOC119729096
NW_024037047.1:35105390..35105408 15/19 Antisense off-target LOC119722451
Publications
???morpholino.displaySummary.papersFirst??? Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016  
???morpholino.displaySummary.papersRecent??? CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae., J Exp Biol 2021  
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