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Echinobase

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ECB-MORPHOLINO-24505898
Morpholino Name: ascl1 MO1
Synonyms: Lv-AcSc
Target mRNA: ascl1
Derived from:

Morpholino Type: translation blocking morpholino
Sequence: 5' ACAATGTTCTCCATTTTGTGTCTTT 3'

Source: Gene Tools LLC


Target mRNA L. variegatus
Identities 24/25
genome
MO
 
5' AAGGACACAAAATGGAGAACATTGT 3'
   || ||||||||||||||||||||||
3' TTTCTGTGTTTTACCTCTTGTAACA 5'
      
Genomic Alignments Position Identity Strand Target Target mRNA
S. purpuratus 5.0 NW_022145614.1:6699922..6699946 21/25 Antisense off-target gmds
NW_022145600.1:15076498..15076522 21/25 Antisense off-target prom1
NW_022145606.1:44646692..44646716 21/25 Sense off-target LOC584260
NW_022145598.1:1691911..1691935 21/25 Antisense unknown
NW_022144778.1:9570..9594 21/25 Antisense unknown
NW_022144973.1:3525..3549 21/25 Antisense unknown
NW_022145018.1:217341..217365 21/25 Sense unknown
NW_022145597.1:12696082..12696106 21/25 Sense unknown
P. miniata 3.0 NW_024037058.1:17498923..17498947 22/25 Antisense off-target LOC119726720
NW_024037069.1:34446537..34446561 21/25 Sense off-target LOC119738290
NW_024037054.1:7433158..7433182 21/25 Sense unknown
NW_024037058.1:3003074..3003098 21/25 Sense unknown
L. variegatus 3.0 chr19:12713103..12713127 24/25 Sense target ascl1
chr4:64294031..64294055 22/25 Sense off-target LOC121414521
chr1:61873181..61873205 21/25 Sense off-target LOC121418974
chr1:67463668..67463692 21/25 Sense off-target LOC121420553
chr12:8801367..8801391 21/25 Sense off-target LOC121425251
chr2:5734230..5734254 21/25 Sense off-target LOC121407145
chr3:57664569..57664593 21/25 Sense off-target LOC121412083
chr4:6070610..6070634 21/25 Antisense off-target LOC121413247
chr5:34018695..34018719 21/25 Sense off-target LOC121415979
chr9:20223619..20223643 21/25 Sense off-target LOC121421297
chr9:20223619..20223643 21/25 Sense off-target LOC121421299
Publications
???morpholino.displaySummary.papersFirst??? Identification of neural transcription factors required for the differentiation of three neuronal subtypes in the sea urchin embryo., Dev Biol 2018  
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