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Echinobase

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ECB-ANTIBODY-26396610

Antibody Name: sfe1 Ab1

Common Name: fertilization envelope protein 1

RRID:
Synonyms: SFE 1

Antigens
Gene: sfe1

Clone Type: Polyclonal
Source:

Antibody
Clone Number
Purification Serum
Isotype isotype unknown
Host Organism rabbit
Echinoderm Reactivity S. purpuratus
Non-Echinoderm Reactivity
Description: Monospecific antibody against a protein specific to the cortical granules and soft fertilization envelopes of sea urchins obtained from cDNA library immunoscreen. Created by the Wessel lab at Brown University in Rhode Island. Also reacts with Lytechinus variegatus.
Immunogen
Name Recombinant SFE1
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Bacterially expressed protein fused to beta-galactosidase generated using cDNA clone with 1.2-kb insert encoding cysteine-rich repeat region. NCBI Accession #: U57753.
Sequence
Reported Usage
immunofluorescence
immunohistochemistry
western blot
Publications
???displayAntibody.papersFirst??? Cortical granule biogenesis is active throughout oogenesis in sea urchins., Development 1994  
???displayAntibody.papersRecent??? SFE1, a constituent of the fertilization envelope in the sea urchin is made by oocytes and contains low-density lipoprotein-receptor-like repeats., Biol Reprod 2000  
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