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Echinobase

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ECB-ANTIBODY-26396013

Antibody Name: acetyl-LOC590586 Ab5

Common Name: acetylated tubulin alpha-1A chain

RRID:
Synonyms: 8E11-6-1, acetylated alpha-tubulin

Antigens
Gene: LOC590586

Clone Type: Monoclonal
Source:

Antibody
Clone Number 8E11-6-1
Purification
Isotype IgG1
Host Organism mouse
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity pig
Description: Reacts with a subset of microtubules in sperm flagellar axonemes. Created by the Linck lab at the University of Minnesota, Minneapolis. Also reacts with molluscs (Spisula solidissima) and Arbacia punctulata.
Immunogen
Name Sarkosyl-resistant protofilament ribbons
Type polypeptide
Post Translational Modifications Acetylated
Source Organism purple sea urchin
Description Protein mixture consisting principally of alpha and beta-tubulin, tektins A, B, and C, and two proteins of about ~77 and ~83 kDa extracted from sperm double microtubles using a 0.5% Sarkosyl solution.
Sequence
Reported Usage
immunofluorescence
western blot
Publications
???displayAntibody.papersFirst??? Evidence for a non-tubulin spindle matrix and for spindle components immunologically related to tektin filaments., J Cell Sci 1992  
???displayAntibody.papersRecent??? Tektins are heterodimeric polymers in flagellar microtubules with axial periodicities matching the tubulin lattice., J Biol Chem 1994  
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