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ECB-ANTIBODY-26396236

Antibody Name: msp130 Ab6

Common Name: mesenchyme-specific cell surface glycoprotein

RRID:
Synonyms: 130-kDa PMC-specific cell surface glycoprotein, 6a9, MSP-130

Antigens
Gene: msp130

Clone Type: Monoclonal
Source:

Antibody
Clone Number 6a9
Purification
Isotype IgM
Host Organism mouse
Echinoderm Reactivity S. purpuratus, P. lividus, L. pictus
Non-Echinoderm Reactivity
Description: PMC-specific antibody that can be utilized to mark skeletogenic cells in sea urchin embryos (Dendraster excentricus, Lytechinus variegatus, etc.). Originally created by Charles A. Ettensohn and Dr. R. McClay (Duke University, Durham, NC). Hybridoma supernatant previously supplied by the Ettensohn lab (Carnegie Mellon University, Pittsburgh, PA).
Immunogen
Name Purified PMCs
Type other immunogen type
Post Translational Modifications unknown post translational modifications
Source Organism variegated sea urchin
Description 2 X 10^6 primary mesenchyme cells (PMCs) purified from mesenchyme blastula stage embryos by panning the cells of wheat germ agglutinin-coated dishes then fixing them.
Sequence
Reported Usage
enzyme-linked immunosorbent assay
immunofluorescence
immunohistochemistry
western blot
Publications
???displayAntibody.papersFirst??? Cell lineage conversion in the sea urchin embryo., Dev Biol 1988  
???displayAntibody.papersRecent??? Ethanol exposure perturbs sea urchin development and disrupts developmental timing., Dev Biol 2023  
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