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Echinobase

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ECB-ANTIBODY-26395892

Antibody Name: LOC590586 Ab3

Common Name: tubulin alpha-1A chain

RRID: AB_477593
Synonyms: DM1A, alpha-tubulin

Antigens
Gene: LOC590586

Clone Type: Monoclonal
Source: Sigma-Aldrich

Antibody
Clone Number DM1A
Purification Ascites
Isotype IgG1
Host Organism mouse
Echinoderm Reactivity S. purpuratus, P. miniata, L. pictus
Non-Echinoderm Reactivity common urchin, cow, human, mouse, rat, yeast
Description: Reacts with a highly conserved primary sequence within C-terminal domain of a ~50 kDa cytoskeletal protein. Antibody originally created and previously provided by Steph H. Blose (Cold Spring Harbor Laboratory) (PMID: 6538204). Currently supplied by Sigma-Aldrich (#T9026). Also reacts with Pisaster ochraceus.
Immunogen
Name Chick Brain Microtubules
Type polypeptide
Post Translational Modifications None
Source Organism chicken
Description Native proteins purified from one-day-old chick brains using temperature-dependent assembly and ion-exchange chromatography.
Sequence
Reported Usage
immunofluorescence
western blot
Publications
???displayAntibody.papersFirst??? Cyclic assembly-disassembly of cortical microtubules during maturation and early development of starfish oocytes., Dev Biol 1984  
???displayAntibody.papersRecent??? Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes., Curr Biol 2021  
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