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Antibody Name: pm27 Ab1 Common Name: 27 kDa primary mesenchyme-specific spicule protein RRID: Synonyms: PM27 Antigens Gene: pm27 Clone Type: Polyclonal Source:

Antibody
Clone Number
Purification	Affinity Purification
Isotype	isotype unknown
Host Organism	rabbit
Echinoderm Reactivity	S. purpuratus
Non-Echinoderm Reactivity
Description:	Skeletogenic mesenchyme-specific antibody created and previously supplied by the laboratory of Professor Rudolph Raff at the University of Washington, Seattle.
Immunogen
Name	PM27-PWR 590 and PM27-pGEX fusion proteins
Type	polypeptide
Post Translational Modifications	None
Source Organism	purple sea urchin
Description	Both fusion proteins contain the same 243 amino-acid coding region, which originated from the nucleotide sequence within the NaeI (+156) and EcoRI (+883) sites of a Bluescript SK+ vector. PM27-PWR 590 was used for the primary injection, while PM27-pGEX fusion protein was used for the final injection.
Sequence
Reported Usage
immunohistochemistry
western blot
Publications
???displayAntibody.papersFirst???	Structure, expression, and extracellular targeting of PM27, a skeletal protein associated specifically with growth of the sea urchin larval spicule., Dev Biol 1995
???displayAntibody.papersRecent???	Expression of spicule matrix proteins in the sea urchin embryo during normal and experimentally altered spiculogenesis., Dev Biol 2000
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