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Echinobase

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ECB-ANTIBODY-26395875

Antibody Name: LOC590586 Ab1

Common Name: tubulin alpha-1A chain

RRID:
Synonyms: 1-2.3, alpha-tubulin

Antigens
Gene: LOC590586

Clone Type: Monoclonal
Source:

Antibody
Clone Number 1-2.3
Purification Immunoglobulin Fractionation
Isotype IgG
Host Organism mouse
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity
Description: Antibody previously demonstrated to immobilize sperm flagella in vitro. Created and previously supplied by Dr. David Asai of Purdue University (PMID: 6892013), who currently has a lab at the Howard Hudges Medical Institute. Also reacts with Strongylocentrotus droebachiensis.
Immunogen
Name Spermatozoan Axoneme Extract
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Protein mixture consisting of dyneins, tubulins, and several other axonemal proteins extracted using potassium chloride.
Sequence
Reported Usage
immunofluorescence
western blot
Publications
???displayAntibody.papersFirst??? Two different monoclonal antibodies to alpha-tubulin inhibit the bending of reactivated sea urchin spermatozoa., Cell Motil 1982  
???displayAntibody.papersRecent??? Sea urchin oocytes possess elaborate cortical arrays of microfilaments, microtubules, and intermediate filaments., Dev Biol 1989  
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