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Echinobase

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ECB-ANTIBODY-26395773

Antibody Name: LOC590586 Ab2

Common Name: tubulin alpha-1A chain

RRID: AB_2210201
Synonyms: Tyr-Tubulin, YL1/2, tyrosinated alpha-tubulin, tyrosylated tubulin

Antigens
Gene: LOC590586

Clone Type: Monoclonal
Source: Thermo Fisher, Sigma-Aldrich

Antibody
Clone Number YL1/2
Purification Affinity Purification
Isotype IgG2a
Host Organism rat
Echinoderm Reactivity S. purpuratus, H. pulcherrimus
Non-Echinoderm Reactivity chicken, dog, fruitfly, human, mouse, pig, rabbit, rat, yeast
Description: Reacts with a highly conserved ~55 kDa microtubule protein in several echinoid and asteroid species. Has several commercial suppliers, including Thermofisher Scientific (#MA1-80017), Sigma-Aldrich (Chemicon, #MAP1864), and BioRad (Serotec Ltd. (Oxford, UK) (#MCA77G)). Originally created in the lab of J. K. Kilmartin (PMID: 6811596).
Immunogen
Name Yeast tubulin
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description Cycle-purified linear protein sequence requiring an aromatic residue at the C-terminus, with two adjacent amino acids being negatively charged (Glu-Glu-Tyr).
Sequence
Reported Usage
immunofluorescence
immunohistochemistry
western blot
Publications
???displayAntibody.papersFirst??? Intermediate filaments, microtubules and microfilaments in epidermis of sea urchin tube foot., Cell Tissue Res 1984  
???displayAntibody.papersRecent??? Building the cytokinetic contractile ring in an early embryo: Initiation as clusters of myosin II, anillin and septin, and visualization of a septin filament network., PLoS One 2021                    
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