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Mar Drugs
2023 Oct 27;2111:. doi: 10.3390/md21110564.
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Genome Sequencing-Based Mining and Characterization of a Novel Alginate Lyase from Vibrio alginolyticus S10 for Specific Production of Disaccharides.
Shu Z
,
Wang G
,
Liu F
,
Xu Y
,
Sun J
,
Hu Y
,
Dong H
,
Zhang J
.
Abstract
Alginate oligosaccharides prepared by alginate lyases attracted great attention because of their desirable biological activities. However, the hydrolysis products are always a mixture of oligosaccharides with different degrees of polymerization, which increases the production cost because of the following purification procedures. In this study, an alginate lyase, Alg4755, with high product specificity was identified, heterologously expressed, and characterized from Vibrio alginolyticus S10, which was isolated from the intestine of sea cucumber. Alg4755 belonged to the PL7 family with two catalytic domains, which was composed of 583 amino acids. Enzymatic characterization results show that the optimal reaction temperature and pH of Alg4755 were 35 °C and 8.0, respectively. Furthermore, Alg4755 was identified to have high thermal and pH stability. Moreover, the final hydrolysis products of sodium alginate catalyzed by Alg4755 were mainly alginate disaccharides with a small amount of alginate trisaccharides. The results demonstrate that alginate lyase Alg4755 could have a broad application prospect because of its high product specificity and desirable catalytic properties.
Akhter,
PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and composition-based strategies.
2012, Pubmed
Akhter,
PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and composition-based strategies.
2012,
Pubmed
Almagro Armenteros,
SignalP 5.0 improves signal peptide predictions using deep neural networks.
2019,
Pubmed
Arntzen,
Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase.
2021,
Pubmed
Badur,
Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic.
2015,
Pubmed
Besemer,
GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions.
2001,
Pubmed
Burge,
Rfam 11.0: 10 years of RNA families.
2013,
Pubmed
Cheng,
Characteristics and applications of alginate lyases: A review.
2020,
Pubmed
Cheng,
Biochemical characterization and degradation pattern analysis of a novel PL-6 alginate lyase from Streptomyces coelicolor A3(2).
2020,
Pubmed
Dong,
Alginate Lyase Aly36B is a New Bacterial Member of the Polysaccharide Lyase Family 36 and Catalyzes by a Novel Mechanism With Lysine as Both the Catalytic Base and Catalytic Acid.
2019,
Pubmed
Dong,
Molecular insight into the role of the N-terminal extension in the maturation, substrate recognition, and catalysis of a bacterial alginate lyase from polysaccharide lyase family 18.
2014,
Pubmed
Fang,
Identification and activation of TLR4-mediated signalling pathways by alginate-derived guluronate oligosaccharide in RAW264.7 macrophages.
2017,
Pubmed
Feng,
Alginate Oligosaccharide Alleviates Monocrotaline-Induced Pulmonary Hypertension via Anti-Oxidant and Anti-Inflammation Pathways in Rats.
2020,
Pubmed
Garron,
Structural and mechanistic classification of uronic acid-containing polysaccharide lyases.
2010,
Pubmed
Jumper,
Highly accurate protein structure prediction with AlphaFold.
2021,
Pubmed
Kim,
Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40.
2012,
Pubmed
Kitamikado,
Two types of bacterial alginate lyases.
1992,
Pubmed
Krzywinski,
Circos: an information aesthetic for comparative genomics.
2009,
Pubmed
Lagesen,
RNAmmer: consistent and rapid annotation of ribosomal RNA genes.
2007,
Pubmed
Li,
Alginate degrading enzymes: an updated comprehensive review of the structure, catalytic mechanism, modification method and applications of alginate lyases.
2021,
Pubmed
Li,
Combined enzymatic hydrolysis and selective fermentation for green production of alginate oligosaccharides from Laminaria japonica.
2019,
Pubmed
Liu,
Alginate Oligosaccharides: Production, Biological Activities, and Potential Applications.
2019,
Pubmed
Lombard,
A hierarchical classification of polysaccharide lyases for glycogenomics.
2010,
Pubmed
Lowe,
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.
1997,
Pubmed
Lu,
CDD/SPARCLE: the conserved domain database in 2020.
2020,
Pubmed
Manni,
BUSCO: Assessing Genomic Data Quality and Beyond.
2021,
Pubmed
Mao,
The depolymerization of sodium alginate by oxidative degradation.
2012,
Pubmed
Matsushima,
Analysis of extracellular alginate lyase and its gene from a marine bacterial strain, Pseudoalteromonas atlantica AR06.
2010,
Pubmed
Ogura,
Substrate recognition by family 7 alginate lyase from Sphingomonas sp. A1.
2008,
Pubmed
Pan,
Alginate Oligosaccharide Ameliorates D-Galactose-Induced Kidney Aging in Mice through Activation of the Nrf2 Signaling Pathway.
2021,
Pubmed
Powell,
Targeted disruption of the extracellular polymeric network of Pseudomonas aeruginosa biofilms by alginate oligosaccharides.
2018,
Pubmed
Qin,
Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family.
2018,
Pubmed
Rodrigues,
Impact of enzyme- and ultrasound-assisted extraction methods on biological properties of red, brown, and green seaweeds from the central west coast of Portugal.
2015,
Pubmed
Sha,
Cloning and Characterization of a Novel Endo-Type Metal-Independent Alginate Lyase from the Marine Bacteria Vibrio sp. Ni1.
2022,
Pubmed
Sun,
Characterization of a Novel Alginate Lyase with Two Alginate Lyase Domains from the Marine Bacterium Vibrio sp. C42.
2022,
Pubmed
Suzuki,
A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner.
2006,
Pubmed
Thomas,
Comparative characterization of two marine alginate lyases from Zobellia galactanivorans reveals distinct modes of action and exquisite adaptation to their natural substrate.
2013,
Pubmed
Tran,
Alginate oligosaccharide (AOS) improves immuno-metabolic systems by inhibiting STOML2 overexpression in high-fat-diet-induced obese zebrafish.
2019,
Pubmed
Walker,
Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement.
2014,
Pubmed
Wang,
Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression.
2019,
Pubmed
Wang,
Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property.
2019,
Pubmed
Wang,
Cloning, Secretory Expression and Characterization of a Unique pH-Stable and Cold-Adapted Alginate Lyase.
2020,
Pubmed
Wick,
Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads.
2017,
Pubmed
Wilkins,
Protein identification and analysis tools in the ExPASy server.
1999,
Pubmed
Wong,
ALGINATE LYASE: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications.
2000,
Pubmed
Xing,
Advances in Research on the Bioactivity of Alginate Oligosaccharides.
2020,
Pubmed
Xu,
Structural and molecular basis for the substrate positioning mechanism of a new PL7 subfamily alginate lyase from the arctic.
2020,
Pubmed
Xu,
Diversity of Three-Dimensional Structures and Catalytic Mechanisms of Alginate Lyases.
2018,
Pubmed
Xue,
A Novel Alginate Lyase: Identification, Characterization, and Potential Application in Alginate Trisaccharide Preparation.
2022,
Pubmed
Yamasaki,
A structural basis for depolymerization of alginate by polysaccharide lyase family-7.
2005,
Pubmed
Yang,
Anti-diabetic effect of oligosaccharides from seaweed Sargassum confusum via JNK-IRS1/PI3K signalling pathways and regulation of gut microbiota.
2019,
Pubmed
Zhang,
Microbiota from alginate oligosaccharide-dosed mice successfully mitigated small intestinal mucositis.
2020,
Pubmed
Zhang,
dbCAN2: a meta server for automated carbohydrate-active enzyme annotation.
2018,
Pubmed
Zhao,
Low-viscosity sodium alginate combined with TiO2 nanoparticles for improving neuroblastoma treatment.
2021,
Pubmed
Zhu,
Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from Flammeovirga sp. NJ-04 and its use for preparation alginate oligosaccharides.
2019,
Pubmed
Zhu,
Cloning and characterization of a new pH-stable alginate lyase with high salt tolerance from marine Vibrio sp. NJ-04.
2018,
Pubmed
Zhuang,
Characterization of a Novel PolyM-Preferred Alginate Lyase from Marine Vibrio splendidus OU02.
2018,
Pubmed
