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Mar Biotechnol (NY)
2023 Dec 01;256:1110-1122. doi: 10.1007/s10126-023-10263-y.
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Metabolome and Transcriptome Association Analysis Reveals the Link Between Pigmentation and Nutrition Utilization in the Juveniles of Sea Cucumber Holothuria leucospilota.
Huang J
,
E Z
,
Pan W
,
Li Z
,
Lin T
,
Ren C
,
Luo P
,
Ma B
,
Liu Y
,
Wu X
,
Hu C
,
Jiang X
,
Chen T
.
Abstract
The sea cucumber Holothuria leucospilota is an economically and ecologically important tropical species. Following development into juveniles, H. leucospilota undergoes a color change from white to black, involving a pigmentation process for over a period of several months. In this study, a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and Next-Generation sequencing (NGS) were employed to investigate the changes in metabolomic and transcriptomic profiles during pigmentation in H. leucospilota juveniles. The metabolomic analysis identified a total of 341 metabolites, of which 52 were found to be differentially regulated (P < 0.05 and VIP > 1), with 27 being upregulated in white individuals and 25 in black individuals. Additionally, 632 differentially expressed genes (DEGs) were identified, with 380 genes upregulated in white samples and 252 genes upregulated in black samples. Interestingly, the melanin content and tyrosinase transcript levels did not display significant differences between the two groups. Metabolomic data suggested the involvement of the linoleic acid metabolic pathway in pigmentation. Transcriptomic analysis, coupled with realtime PCR validation, revealed a decrease in the transcript levels of digestive enzymes like α-amylase, maltase-glucoamylase, and trehalase after the juveniles changed to black. Furthermore, the mRNA expressions of major yolk proteins showed a decline, indicating a shift in the accumulation of protein nutrient sources. Overall, our findings suggest that during the pigmentation process in H. leucospilota, no significant changes were observed in the classical melanin pathway, while notable alterations were observed in their nutritional status. This study provides valuable insights into the regulatory mechanisms of pigmentation in marine organisms.
2018YFD0901605 National Key R & D Program of China, 2022YFD2401301 National Key R & D Program of China, 41906101 National Natural Science Foundation of China, 42176132 National Natural Science Foundation of China, COMS2020Q03 Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences
Abdelrazig,
Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling.
2020, Pubmed
Abdelrazig,
Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling.
2020,
Pubmed
Brooks,
The major yolk protein in sea urchins is a transferrin-like, iron binding protein.
2002,
Pubmed
,
Echinobase
Calestani,
These Colors Don't Run: Regulation of Pigment-Biosynthesis in Echinoderms.
2018,
Pubmed
,
Echinobase
Chen,
The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins.
2023,
Pubmed
,
Echinobase
Eriksson,
Sea cucumber aquaculture in the Western Indian ocean: challenges for sustainable livelihood and stock improvement.
2012,
Pubmed
,
Echinobase
Gagnebin,
Metabolomic analysis of urine samples by UHPLC-QTOF-MS: Impact of normalization strategies.
2017,
Pubmed
Hiramatsu,
Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins.
2015,
Pubmed
Horai,
MassBank: a public repository for sharing mass spectral data for life sciences.
2010,
Pubmed
Huo,
First echinoderm trehalase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning and mRNA expression in different tissues, embryonic and larval stages, and under a starvation challenge.
2018,
Pubmed
,
Echinobase
Kanehisa,
KEGG: kyoto encyclopedia of genes and genomes.
2000,
Pubmed
Kang,
Allele-specific PCR genotyping of the HSP70 gene polymorphism discriminating the green and red color variants sea cucumber (Apostichopus japonicus).
2011,
Pubmed
,
Echinobase
Kumar,
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.
2016,
Pubmed
Marrugo-Negrete,
Sea Cucumber as Bioindicator of Trace Metal Pollution in Coastal Sediments.
2021,
Pubmed
,
Echinobase
Navarro-Reig,
Evaluation of changes induced in rice metabolome by Cd and Cu exposure using LC-MS with XCMS and MCR-ALS data analysis strategies.
2015,
Pubmed
Ni,
Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17β.
2014,
Pubmed
Nicholson,
Metabonomics: a platform for studying drug toxicity and gene function.
2002,
Pubmed
Oskouian,
Death and taxis: what non-mammalian models tell us about sphingosine-1-phosphate.
2004,
Pubmed
Shyu,
Expression of the vitellogenin gene in female and male sea urchin.
1986,
Pubmed
,
Echinobase
Slominski,
Melanin pigmentation in mammalian skin and its hormonal regulation.
2004,
Pubmed
Sud,
LMSD: LIPID MAPS structure database.
2007,
Pubmed
Thévenot,
Analysis of the Human Adult Urinary Metabolome Variations with Age, Body Mass Index, and Gender by Implementing a Comprehensive Workflow for Univariate and OPLS Statistical Analyses.
2015,
Pubmed
Tian,
What makes an egg unique? Clues from evolutionary scenarios of egg-specific genes.
2010,
Pubmed
Tufail,
Molecular characteristics of insect vitellogenins.
2008,
Pubmed
Unuma,
The major yolk protein is synthesized in the digestive tract and secreted into the body cavities in sea urchin larvae.
2009,
Pubmed
,
Echinobase
Wishart,
HMDB: the Human Metabolome Database.
2007,
Pubmed
Xia,
Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst.
2011,
Pubmed
