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Physiol Mol Biol Plants
2023 Feb 01;292:159-172. doi: 10.1007/s12298-023-01288-7.
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Dynamics of the sucrose metabolism and related gene expression in tomato fruits under water deficit.
Barbosa ACO
,
Rocha DS
,
Silva GCB
,
Santos MGM
,
Camillo LR
,
de Oliveira PHGA
,
Cavalari AA
,
Costa MGC
.
Abstract
UNLABELLED: The impact of water deficit on sucrose metabolism in sink organs like the fruit remains poorly known despite the need to improve fruit crops resilience to drought in the face of climate change. The present study investigated the effects of water deficit on sucrose metabolism and related gene expression in tomato fruits, aiming to identify candidate genes for improving fruit quality upon low water availability. Tomato plants were subjected to irrigated control and water deficit (-60% water supply compared to control) treatments, which were applied from the first fruit set to first fruit maturity stages. The results have shown that water deficit significantly reduced fruit dry biomass and number, among other plant physiological and growth variables, but substantially increased the total soluble solids content. The determination of soluble sugars on the basis of fruit dry weight revealed an active accumulation of sucrose and concomitant reduction in glucose and fructose levels in response to water deficit. The complete repertoire of genes encoding sucrose synthase (SUSY1-7), sucrose-phosphate synthase (SPS1-4), and cytosolic (CIN1-8), vacuolar (VIN1-2) and cell wall invertases (WIN1-4) was identified and characterized, of which SlSUSY4, SlSPS1, SlCIN3, SlVIN2, and SlCWIN2 were shown to be positively regulated by water deficit. Collectively, these results show that water deficit regulates positively the expression of certain genes from different gene families related to sucrose metabolism in fruits, favoring the active accumulation of sucrose in this organ under water-limiting conditions.
SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-023-01288-7.
Almadanim,
Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose-phosphate synthase and is required for a proper cold stress response.
2017, Pubmed
Almadanim,
Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose-phosphate synthase and is required for a proper cold stress response.
2017,
Pubmed
An,
Identification and characterization of the Populus sucrose synthase gene family.
2014,
Pubmed
Andersen,
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.
2004,
Pubmed
Baud,
Structure and expression profile of the sucrose synthase multigene family in Arabidopsis.
2004,
Pubmed
Bilska-Kos,
Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus × giganteus and Zea mays at low temperature.
2020,
Pubmed
Braun,
Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security.
2014,
Pubmed
Castellarin,
Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit.
2007,
Pubmed
Castleden,
Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses.
2004,
Pubmed
Garchery,
A diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit.
2013,
Pubmed
Geigenberger,
Decreased expression of sucrose phosphate synthase strongly inhibits the water stress-induced synthesis of sucrose in growing potato tubers.
1999,
Pubmed
Godt,
Regulation and tissue-specific distribution of mRNAs for three extracellular invertase isoenzymes of tomato suggests an important function in establishing and maintaining sink metabolism.
1997,
Pubmed
Granot,
Substantial roles of hexokinase and fructokinase in the effects of sugars on plant physiology and development.
2014,
Pubmed
Guo,
[GSDS: a gene structure display server].
2007,
Pubmed
Hernandez-Garcia,
Identification and validation of promoters and cis-acting regulatory elements.
2014,
Pubmed
Hou,
Responses of water accumulation and solute metabolism in tomato fruit to water scarcity and implications for main fruit quality variables.
2020,
Pubmed
Ji,
Structure, evolution, and expression of the two invertase gene families of rice.
2005,
Pubmed
Jia,
Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening.
2013,
Pubmed
Jia,
OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.).
2008,
Pubmed
Jiang,
Sucrose metabolism gene families and their biological functions.
2015,
Pubmed
Juárez-Colunga,
Genome-wide analysis of the invertase gene family from maize.
2018,
Pubmed
Kumar,
Vegetable Grafting as a Tool to Improve Drought Resistance and Water Use Efficiency.
2017,
Pubmed
Le Roy,
Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase.
2007,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Lunn,
New complexities in the synthesis of sucrose.
2003,
Pubmed
Lutfiyya,
Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants.
2007,
Pubmed
Meissner,
Mapping quantitative trait loci for freezing tolerance in a recombinant inbred line population of Arabidopsis thaliana accessions Tenela and C24 reveals REVEILLE1 as negative regulator of cold acclimation.
2013,
Pubmed
Mou,
Transcriptomic Analysis Reveals Possible Influences of ABA on Secondary Metabolism of Pigments, Flavonoids and Antioxidants in Tomato Fruit during Ripening.
2015,
Pubmed
Nguyen-Quoc,
A role for 'futile cycles' involving invertase and sucrose synthase in sucrose metabolism of tomato fruit.
2001,
Pubmed
Pagliuso,
Correlation of Apiose Levels and Growth Rates in Duckweeds.
2018,
Pubmed
Qi,
AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis.
2007,
Pubmed
Ripoll,
Water deficit effects on tomato quality depend on fruit developmental stage and genotype.
2016,
Pubmed
Ripoll,
Water shortage and quality of fleshy fruits--making the most of the unavoidable.
2014,
Pubmed
Roitsch,
Function and regulation of plant invertases: sweet sensations.
2004,
Pubmed
Ruan,
Sucrose metabolism: gateway to diverse carbon use and sugar signaling.
2014,
Pubmed
Ruan,
Expression of sucrose synthase in the developing endosperm is essential for early seed development in cotton.
2008,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Scholander,
Sap Pressure in Vascular Plants: Negative hydrostatic pressure can be measured in plants.
1965,
Pubmed
Seneweera,
Diurnal Regulation of Leaf Blade Elongation in Rice by CO2 (Is it Related to Sucrose-Phosphate Synthase Activity?).
1995,
Pubmed
Siebeneichler,
The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
2020,
Pubmed
Stein,
Plant Fructokinases: Evolutionary, Developmental, and Metabolic Aspects in Sink Tissues.
2018,
Pubmed
Sun,
Decrease in leaf sucrose synthesis leads to increased leaf starch turnover and decreased RuBP regeneration-limited photosynthesis but not Rubisco-limited photosynthesis in Arabidopsis null mutants of SPSA1.
2011,
Pubmed
Terry,
Effect of water deficit irrigation and inoculation with Botrytis cinerea on strawberry (Fragaria x ananassa) fruit quality.
2007,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tomato Genome Consortium,
The tomato genome sequence provides insights into fleshy fruit evolution.
2012,
Pubmed
Toroser,
Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves.
1997,
Pubmed
Vargas,
Differential expression of alkaline and neutral invertases in response to environmental stresses: characterization of an alkaline isoform as a stress-response enzyme in wheat leaves.
2007,
Pubmed
Wan,
Evolution of Sucrose Metabolism: The Dichotomy of Invertases and Beyond.
2018,
Pubmed
Wang,
New insights into roles of cell wall invertase in early seed development revealed by comprehensive spatial and temporal expression patterns of GhCWIN1 in cotton.
2012,
Pubmed
Wang,
Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns.
2015,
Pubmed
Welham,
A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus.
2009,
Pubmed
Winter,
Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes.
2000,
Pubmed
Xiang,
Exploring the neutral invertase-oxidative stress defence connection in Arabidopsis thaliana.
2011,
Pubmed
Xiao,
Structure and expression profile of the sucrose synthase gene family in the rubber tree: indicative of roles in stress response and sucrose utilization in the laticifers.
2014,
Pubmed
Yoshida,
Insights into ABA-mediated regulation of guard cell primary metabolism revealed by systems biology approaches.
2019,
Pubmed
Zhang,
Chilling-induced tomato flavor loss is associated with altered volatile synthesis and transient changes in DNA methylation.
2016,
Pubmed
Zhu,
Genome-Wide Analysis of the Sucrose Synthase Gene Family in Grape (Vitis vinifera): Structure, Evolution, and Expression Profiles.
2017,
Pubmed
Zhu,
Sucrose Accumulation in the Sugarcane Stem Is Regulated by the Difference between the Activities of Soluble Acid Invertase and Sucrose Phosphate Synthase.
1997,
Pubmed
