Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Microorganisms
2023 Feb 07;112:. doi: 10.3390/microorganisms11020416.
Show Gene links
Show Anatomy links
Gut Microbiota and Metabolites May Play a Crucial Role in Sea Cucumber Apostichopus japonicus Aestivation.
Kang YH
,
Yang BT
,
Hu RG
,
Zhang P
,
Gu M
,
Cong W
.
Abstract
The constant increase in temperatures under global warming has led to a prolonged aestivation period for Apostichopus japonicus, resulting in considerable losses in production and economic benefits. However, the specific mechanism of aestivation has not been fully elucidated. In this study, we first tried to illustrate the biological mechanisms of aestivation from the perspective of the gut microbiota and metabolites. Significant differences were found in the gut microbiota of aestivating adult A. japonicus (AAJSD group) compared with nonaestivating adult A. japonicus (AAJRT group) and young A. japonicus (YAJRT and YAJSD groups) based on 16S rRNA gene high-throughput sequencing analysis. The abundances of Desulfobacterota, Myxococcota, Bdellovibrionota, and Firmicutes (4 phyla) in the AAJSD group significantly increased. Moreover, the levels of Pseudoalteromonas, Fusibacter, Labilibacter, Litorilituus, Flammeovirga, Polaribacter, Ferrimonas, PB19, and Blfdi19 genera were significantly higher in the AAJSD group than in the other three groups. Further analysis of the LDA effect size showed that species with significant variation in abundance in the AAJSD group, including the phylum Firmicutes and the genera Litorilituus, Fusibacter, and Abilibacter, might be important biomarkers for aestivating adult A. japonicus. In addition, the results of metabolomics analysis showed that there were three distinct metabolic pathways, namely biosynthesis of secondary metabolites, tryptophan metabolism, and sesquiterpenoid and triterpenoid biosynthesis in the AAJSD group compared with the other three groups. Notably, 5-hydroxytryptophan was significantly upregulated in the AAJSD group in the tryptophan metabolism pathway. Moreover, the genera Labilibacter, Litorilituus, Ferrimonas, Flammeovirga, Blfdi19, Fusibacter, Pseudoalteromonas, and PB19 with high abundance in the gut of aestivating adult A. japonicus were positively correlated with the metabolite 5-HTP. These findings suggest that there may be potential biological associations among the gut microbiota, metabolites, and aestivation in A. japonicus. This work may provide a new perspective for further understanding the aestivation mechanism of A. japonicus.
Agus,
Gut Microbiota Regulation of Tryptophan Metabolism in Health and Disease.
2018, Pubmed
Agus,
Gut Microbiota Regulation of Tryptophan Metabolism in Health and Disease.
2018,
Pubmed
Alexeev,
Microbiota-Derived Indole Metabolites Promote Human and Murine Intestinal Homeostasis through Regulation of Interleukin-10 Receptor.
2018,
Pubmed
Caporaso,
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample.
2011,
Pubmed
Chen,
The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber.
2018,
Pubmed
,
Echinobase
Chen,
Comparative phosphoproteomic analysis of intestinal phosphorylated proteins in active versus aestivating sea cucumbers.
2016,
Pubmed
,
Echinobase
Clarke,
The microbiome-gut-brain axis during early life regulates the hippocampal serotonergic system in a sex-dependent manner.
2013,
Pubmed
Cui,
Gut digestion of earthworms significantly attenuates cell-free and -associated antibiotic resistance genes in excess activated sludge by affecting bacterial profiles.
2019,
Pubmed
Gao,
Genome-wide comparative analysis of DNAJ genes and their co-expression patterns with HSP70s in aestivation of the sea cucumber Apostichopus japonicus.
2022,
Pubmed
,
Echinobase
Gao,
Tryptophan Metabolism: A Link Between the Gut Microbiota and Brain.
2020,
Pubmed
Gehrke,
Red Blood Cell Metabolic Responses to Torpor and Arousal in the Hibernator Arctic Ground Squirrel.
2019,
Pubmed
Gheorghe,
Focus on the essentials: tryptophan metabolism and the microbiome-gut-brain axis.
2019,
Pubmed
Haas,
Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.
2011,
Pubmed
Hiong,
Differential gene expression in the brain of the African lungfish, Protopterus annectens, after six days or six months of aestivation in air.
2013,
Pubmed
Hubbard,
Indole and Tryptophan Metabolism: Endogenous and Dietary Routes to Ah Receptor Activation.
2015,
Pubmed
Kaur,
Tryptophan Metabolism by Gut Microbiome and Gut-Brain-Axis: An in silico Analysis.
2019,
Pubmed
Kwon,
Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis.
2019,
Pubmed
Li,
Sea cucumber genome provides insights into saponin biosynthesis and aestivation regulation.
2018,
Pubmed
,
Echinobase
Li,
The Role of Microbiome in Insomnia, Circadian Disturbance and Depression.
2018,
Pubmed
Li,
Signatures within esophageal microbiota with progression of esophageal squamous cell carcinoma.
2020,
Pubmed
Magoč,
FLASH: fast length adjustment of short reads to improve genome assemblies.
2011,
Pubmed
Matenchuk,
Sleep, circadian rhythm, and gut microbiota.
2020,
Pubmed
Meloni,
Preliminary finding of a randomized, double-blind, placebo-controlled, crossover study to evaluate the safety and efficacy of 5-hydroxytryptophan on REM sleep behavior disorder in Parkinson's disease.
2022,
Pubmed
Ogawa,
Gut microbiota depletion by chronic antibiotic treatment alters the sleep/wake architecture and sleep EEG power spectra in mice.
2020,
Pubmed
Popova,
Involvement of brain tryptophan hydroxylase in the mechanism of hibernation.
1993,
Pubmed
Segata,
Metagenomic biomarker discovery and explanation.
2011,
Pubmed
Sellick,
Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling.
2011,
Pubmed
Sen,
Microbiota and sleep: awakening the gut feeling.
2021,
Pubmed
Smith,
Gut microbiome diversity is associated with sleep physiology in humans.
2019,
Pubmed
Szczepanik,
Melatonin and its influence on immune system.
2007,
Pubmed
Wang,
A potential antiapoptotic regulation: The interaction of heat shock protein 70 and apoptosis-inducing factor mitochondrial 1 during heat stress and aestivation in sea cucumber.
2018,
Pubmed
,
Echinobase
Wen,
metaX: a flexible and comprehensive software for processing metabolomics data.
2017,
Pubmed
Xia,
Using MetaboAnalyst 3.0 for Comprehensive Metabolomics Data Analysis.
2016,
Pubmed
Yang,
Genome-Wide DNA Methylation Signatures of Sea Cucumber Apostichopus japonicus during Environmental Induced Aestivation.
2020,
Pubmed
,
Echinobase
Yano,
Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis.
2015,
Pubmed
Youssef,
Comparison of species richness estimates obtained using nearly complete fragments and simulated pyrosequencing-generated fragments in 16S rRNA gene-based environmental surveys.
2009,
Pubmed
Yuan,
A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue.
2012,
Pubmed
Zelante,
Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22.
2013,
Pubmed
