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Mar Drugs
2023 Feb 24;213:. doi: 10.3390/md21030145.
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Glycoproteins Involved in Sea Urchin Temporary Adhesion.
Ventura I
,
Harman V
,
Beynon RJ
,
Santos R
.
Abstract
Biomedical adhesives, despite having been used increasingly in recent years, still face a major technological challenge: strong adhesion in wet environments. In this context, biological adhesives secreted by marine invertebrates have appealing characteristics to incorporate into new underwater biomimetic adhesives: water resistance, nontoxicity and biodegradability. Little is still known about temporary adhesion. Recently, a transcriptomic differential analysis of sea urchin Paracentrotus lividus tube feet pinpointed 16 adhesive/cohesive protein candidates. In addition, it has been demonstrated that the adhesive secreted by this species is composed of high molecular weight proteins associated with N-Acetylglucosamine in a specific chitobiose arrangement. As a follow-up, we aimed to investigate which of these adhesive/cohesive protein candidates were glycosylated through lectin pulldowns, protein identification by mass spectroscopy and in silico characterization. We demonstrate that at least five of the previously identified protein adhesive/cohesive candidates are glycoproteins. We also report the involvement of a third Nectin variant, the first adhesion-related protein to be identified in P. lividus. By providing a deeper characterization of these adhesive/cohesive glycoproteins, this work advances our understanding of the key features that should be replicated in future sea urchin-inspired bioadhesives.
Algrain,
In the footsteps of sea stars: deciphering the catalogue of proteins involved in underwater temporary adhesion.
2022, Pubmed,
Echinobase
Algrain,
In the footsteps of sea stars: deciphering the catalogue of proteins involved in underwater temporary adhesion.
2022,
Pubmed
,
Echinobase
Almagro Armenteros,
SignalP 5.0 improves signal peptide predictions using deep neural networks.
2019,
Pubmed
Anand,
Computational modelling of wet adhesive mussel foot proteins (Bivalvia): Insights into the evolutionary convolution in diverse perspectives.
2020,
Pubmed
Baldwin,
Protein identification by mass spectrometry: issues to be considered.
2004,
Pubmed
Bertemes,
Sticking Together an Updated Model for Temporary Adhesion.
2022,
Pubmed
Bhattacharjee,
Position-specific propensities of amino acids in the β-strand.
2010,
Pubmed
Budisa,
Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine.
2019,
Pubmed
Costa,
Phylogenetic analysis and homology modelling of Paracentrotus lividus nectin.
2010,
Pubmed
,
Echinobase
Davey,
Omics-based molecular analyses of adhesion by aquatic invertebrates.
2021,
Pubmed
Davey,
Transcriptional characterisation of the Exaiptasia pallida pedal disc.
2019,
Pubmed
DeMartini,
A cohort of new adhesive proteins identified from transcriptomic analysis of mussel foot glands.
2017,
Pubmed
Dill,
The protein folding problem.
2008,
Pubmed
Domínguez-Martín,
Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcusmarinus SS120.
2017,
Pubmed
Domínguez-Pérez,
The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes.
2020,
Pubmed
Dreanno,
An alpha2-macroglobulin-like protein is the cue to gregarious settlement of the barnacle Balanus amphitrite.
2006,
Pubmed
Dyson,
The role of hydrophobic interactions in initiation and propagation of protein folding.
2006,
Pubmed
Federle,
Dynamic biological adhesion: mechanisms for controlling attachment during locomotion.
2019,
Pubmed
Floriolli,
Marine Surfaces and the Expression of Specific Byssal Adhesive Protein Variants in Mytilus.
2000,
Pubmed
Gantayet,
Novel proteins identified in the insoluble byssal matrix of the freshwater zebra mussel.
2014,
Pubmed
Hennebert,
An integrated transcriptomic and proteomic analysis of sea star epidermal secretions identifies proteins involved in defense and adhesion.
2015,
Pubmed
,
Echinobase
Hennebert,
Characterisation of the carbohydrate fraction of the temporary adhesive secreted by the tube feet of the sea star Asterias rubens.
2011,
Pubmed
,
Echinobase
Hennebert,
Sea star tenacity mediated by a protein that fragments, then aggregates.
2014,
Pubmed
,
Echinobase
Johnson,
NCBI BLAST: a better web interface.
2008,
Pubmed
Jumper,
Highly accurate protein structure prediction with AlphaFold.
2021,
Pubmed
Kang,
Molecular insights into the powerful mucus-based adhesion of limpets (Patella vulgata L.).
2020,
Pubmed
,
Echinobase
Lebesgue,
Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach.
2016,
Pubmed
,
Echinobase
Lefevre,
Sea star-inspired recombinant adhesive proteins self-assemble and adsorb on surfaces in aqueous environments to form cytocompatible coatings.
2020,
Pubmed
,
Echinobase
Lengerer,
Biological adhesion of the flatworm Macrostomum lignano relies on a duo-gland system and is mediated by a cell type-specific intermediate filament protein.
2014,
Pubmed
Lengerer,
Adhesive organ regeneration in Macrostomum lignano.
2016,
Pubmed
Lengerer,
Properties of temporary adhesion systems of marine and freshwater organisms.
2018,
Pubmed
Lengerer,
The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa.
2018,
Pubmed
,
Echinobase
Lengerer,
Interspecies comparison of sea star adhesive proteins.
2019,
Pubmed
,
Echinobase
Li,
Databases and Bioinformatic Tools for Glycobiology and Glycoproteomics.
2020,
Pubmed
Lin,
Histology and transcriptomic analyses of barnacles with different base materials and habitats shed lights on the duplication and chemical diversification of barnacle cement proteins.
2021,
Pubmed
Liu,
Comparative proteomics for an in-depth understanding of bioadhesion mechanisms and evolution across metazoans.
2022,
Pubmed
Lu,
Adhesion of mussel foot proteins to different substrate surfaces.
2013,
Pubmed
Lu,
CDD/SPARCLE: the conserved domain database in 2020.
2020,
Pubmed
Madeira,
Search and sequence analysis tools services from EMBL-EBI in 2022.
2022,
Pubmed
Matranga,
A new extracellular matrix protein of the sea urchin embryo with properties of a substrate adhesion molecule.
1992,
Pubmed
,
Echinobase
Mitchell,
InterPro in 2019: improving coverage, classification and access to protein sequence annotations.
2019,
Pubmed
Ohkawa,
A glycosylated byssal precursor protein from the green mussel Perna viridis with modified dopa side-chains.
2004,
Pubmed
Pandey,
Mussel-inspired bioadhesives in healthcare: design parameters, current trends, and future perspectives.
2020,
Pubmed
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Pjeta,
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin Paracentrotus lividus.
2020,
Pubmed
,
Echinobase
Pjeta,
Temporary adhesion of the proseriate flatworm Minona ileanae.
2019,
Pubmed
Rees,
Fingerprinting of Proteins that Mediate Quagga Mussel Adhesion using a De Novo Assembled Foot Transcriptome.
2019,
Pubmed
Rittschof,
Crustacean peptide and peptide-like pheromones and kairomones.
2004,
Pubmed
Rodrigues,
Profiling of adhesive-related genes in the freshwater cnidarian Hydra magnipapillata by transcriptomics and proteomics.
2016,
Pubmed
Santos,
First insights into the biochemistry of tube foot adhesive from the sea urchin Paracentrotus lividus (Echinoidea, Echinodermata).
2009,
Pubmed
,
Echinobase
Santos,
Mapping sea urchins tube feet proteome--a unique hydraulic mechano-sensory adhesive organ.
2013,
Pubmed
,
Echinobase
Schwartz,
Serine substitutions are linked to codon usage and differ for variable and conserved protein regions.
2019,
Pubmed
Senior,
Improved protein structure prediction using potentials from deep learning.
2020,
Pubmed
Stewart,
Natural Underwater Adhesives.
2011,
Pubmed
Stewart,
The role of coacervation and phase transitions in the sandcastle worm adhesive system.
2017,
Pubmed
Tabb,
Verification of automated peptide identifications from proteomic tandem mass spectra.
2006,
Pubmed
Thompson,
Optimization of the enzyme-linked lectin assay for enhanced glycoprotein and glycoconjugate analysis.
2011,
Pubmed
Tian,
Transcriptomes reveal the involved genes in the sea urchin Mesocentrotus nudus exposed to high flow velocities.
2022,
Pubmed
,
Echinobase
Toubarro,
Cloning, Characterization, and Expression Levels of the Nectin Gene from the Tube Feet of the Sea Urchin Paracentrotus Lividus.
2016,
Pubmed
,
Echinobase
Urushida,
Identification and functional characterization of a novel barnacle cement protein.
2007,
Pubmed
Viana,
Nanoscale characterization of the temporary adhesive of the sea urchin Paracentrotus lividus.
2018,
Pubmed
,
Echinobase
Wudarski,
The free-living flatworm Macrostomum lignano.
2020,
Pubmed
Wunderer,
A mechanism for temporary bioadhesion.
2019,
Pubmed
Zeng,
Papillae revisited and the nature of the adhesive secreting collocytes.
2019,
Pubmed
Zhao,
Glycosylated hydroxytryptophan in a mussel adhesive protein from Perna viridis.
2009,
Pubmed
Zhao,
Probing the adhesive footprints of Mytilus californianus byssus.
2006,
Pubmed
