ECB-ART-51165
IUCrJ
2022 Nov 01;9Pt 6:778-791. doi: 10.1107/S2052252522010193.
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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Zielinski KA
,
Prester A
,
Andaleeb H
,
Bui S
,
Yefanov O
,
Catapano L
,
Henkel A
,
Wiedorn MO
,
Lorbeer O
,
Crosas E
,
Meyer J
,
Mariani V
,
Domaracky M
,
White TA
,
Fleckenstein H
,
Sarrou I
,
Werner N
,
Betzel C
,
Rohde H
,
Aepfelbacher M
,
Chapman HN
,
Perbandt M
,
Steiner RA
,
Oberthuer D
.
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Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.
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MC_UP_A025_1012 Medical Research Council
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