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ECB-ART-51165
IUCrJ 2022 Nov 01;9Pt 6:778-791. doi: 10.1107/S2052252522010193.
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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.

Zielinski KA , Prester A , Andaleeb H , Bui S , Yefanov O , Catapano L , Henkel A , Wiedorn MO , Lorbeer O , Crosas E , Meyer J , Mariani V , Domaracky M , White TA , Fleckenstein H , Sarrou I , Werner N , Betzel C , Rohde H , Aepfelbacher M , Chapman HN , Perbandt M , Steiner RA , Oberthuer D .


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Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

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References [+] :
Adams, PHENIX: a comprehensive Python-based system for macromolecular structure solution. 2010, Pubmed