Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Development
2022 Nov 15;14922:. doi: 10.1242/dev.200982.
Show Gene links
Show Anatomy links
Single-cell RNA-sequencing analysis of early sea star development.
Foster S
,
Oulhen N
,
Fresques T
,
Zaki H
,
Wessel G
.
???displayArticle.abstract???
Echinoderms represent a broad phylum with many tractable features to test evolutionary changes and constraints. Here, we present a single-cell RNA-sequencing analysis of early development in the sea star Patiria miniata, to complement the recent analysis of two sea urchin species. We identified 20 cell states across six developmental stages from 8 hpf to mid-gastrula stage, using the analysis of 25,703 cells. The clusters were assigned cell states based on known marker gene expression and by in situ RNA hybridization. We found that early (morula, 8-14 hpf) and late (blastula-to-mid-gastrula) cell states are transcriptionally distinct. Cells surrounding the blastopore undergo rapid cell state changes that include endomesoderm diversification. Of particular import to understanding germ cell specification is that we never see Nodal pathway members within Nanos/Vasa-positive cells in the region known to give rise to the primordial germ cells (PGCs). The results from this work contrast the results of PGC specification in the sea urchin, and the dataset presented here enables deeper comparative studies in tractable developmental models for testing a variety of developmental mechanisms.
Annunziata,
Intact cluster and chordate-like expression of ParaHox genes in a sea star.
2013, Pubmed,
Echinobase
Annunziata,
Intact cluster and chordate-like expression of ParaHox genes in a sea star.
2013,
Pubmed
,
Echinobase
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018,
Pubmed
Calestani,
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening.
2003,
Pubmed
,
Echinobase
Cary,
Systematic comparison of sea urchin and sea star developmental gene regulatory networks explains how novelty is incorporated in early development.
2020,
Pubmed
,
Echinobase
Chan,
Molecular recording of mammalian embryogenesis.
2019,
Pubmed
Cheatle Jarvela,
A gene regulatory network for apical organ neurogenesis and its spatial control in sea star embryos.
2016,
Pubmed
,
Echinobase
Duboc,
Left-right asymmetry in the sea urchin embryo is regulated by nodal signaling on the right side.
2005,
Pubmed
,
Echinobase
Extavour,
Mechanisms of germ cell specification across the metazoans: epigenesis and preformation.
2003,
Pubmed
Foster,
A single cell RNA sequencing resource for early sea urchin development.
2020,
Pubmed
,
Echinobase
Fresques,
Nodal induces sequential restriction of germ cell factors during primordial germ cell specification.
2018,
Pubmed
,
Echinobase
Fresques,
Selective accumulation of germ-line associated gene products in early development of the sea star and distinct differences from germ-line development in the sea urchin.
2014,
Pubmed
,
Echinobase
Fresques,
The diversity of nanos expression in echinoderm embryos supports different mechanisms in germ cell specification.
2016,
Pubmed
,
Echinobase
Fujii,
Role of the nanos homolog during sea urchin development.
2009,
Pubmed
,
Echinobase
Fujiwara,
Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage.
1994,
Pubmed
Gildor,
Regulatory heterochronies and loose temporal scaling between sea star and sea urchin regulatory circuits.
2017,
Pubmed
,
Echinobase
Hibino,
The immune gene repertoire encoded in the purple sea urchin genome.
2006,
Pubmed
,
Echinobase
Hinman,
Embryonic neurogenesis in echinoderms.
2018,
Pubmed
,
Echinobase
Hinman,
Expression of a gene encoding a Gata transcription factor during embryogenesis of the starfish Asterina miniata.
2003,
Pubmed
,
Echinobase
Hinman,
Evolutionary plasticity of developmental gene regulatory network architecture.
2007,
Pubmed
,
Echinobase
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Johnson,
Regulative germ cell specification in axolotl embryos: a primitive trait conserved in the mammalian lineage.
2003,
Pubmed
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Korsunsky,
Fast, sensitive and accurate integration of single-cell data with Harmony.
2019,
Pubmed
Macosko,
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.
2015,
Pubmed
Massri,
Developmental single-cell transcriptomics in the Lytechinus variegatus sea urchin embryo.
2021,
Pubmed
,
Echinobase
Materna,
Notch and Nodal control forkhead factor expression in the specification of multipotent progenitors in sea urchin.
2013,
Pubmed
,
Echinobase
McCauley,
A conserved gene regulatory network subcircuit drives different developmental fates in the vegetal pole of highly divergent echinoderm embryos.
2010,
Pubmed
,
Echinobase
McCauley,
Expression of wnt and frizzled genes during early sea star development.
2013,
Pubmed
,
Echinobase
Nishihira,
Macrophage migration inhibitory factor (MIF): its essential role in the immune system and cell growth.
2000,
Pubmed
Oulhen,
Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.
2019,
Pubmed
,
Echinobase
Paganos,
Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome.
2021,
Pubmed
,
Echinobase
Perillo,
A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.
2022,
Pubmed
,
Echinobase
Rafiq,
The genomic regulatory control of skeletal morphogenesis in the sea urchin.
2012,
Pubmed
,
Echinobase
Saitou,
Specification of germ cell fate in mice.
2003,
Pubmed
Stuart,
Comprehensive Integration of Single-Cell Data.
2019,
Pubmed
Sun,
Signal-dependent regulation of the sea urchin skeletogenic gene regulatory network.
2014,
Pubmed
,
Echinobase
Yajima,
Small micromeres contribute to the germline in the sea urchin.
2011,
Pubmed
,
Echinobase
Yankura,
Gene regulatory network for neurogenesis in a sea star embryo connects broad neural specification and localized patterning.
2013,
Pubmed
,
Echinobase
