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Echinobase
ECB-ART-48389
Sci Rep 2018 Oct 30;81:16029. doi: 10.1038/s41598-018-34038-x.
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The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development.

Espiritu EB , Crunk AE , Bais A , Hochbaum D , Cervino AS , Phua YL , Butterworth MB , Goto T , Ho J , Hukriede NA , Cirio MC .


Abstract
The molecular events driving specification of the kidney have been well characterized. However, how the initial kidney field size is established, patterned, and proportioned is not well characterized. Lhx1 is a transcription factor expressed in pronephric progenitors and is required for specification of the kidney, but few Lhx1 interacting proteins or downstream targets have been identified. By tandem-affinity purification, we isolated FRY like transcriptional coactivator (Fryl), one of two paralogous genes, fryl and furry (fry), have been described in vertebrates. Both proteins were found to interact with the Ldb1-Lhx1 complex, but our studies focused on Lhx1/Fry functional roles, as they are expressed in overlapping domains. We found that Xenopus embryos depleted of fry exhibit loss of pronephric mesoderm, phenocopying the Lhx1-depleted animals. In addition, we demonstrated a synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney. Therefore, our data shows that a constitutively-active Ldb1-Lhx1 complex interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.

PubMed ID: 30375416
PMC ID: PMC6207768
Article link: Sci Rep
Grant support: [+]

Species referenced: Echinodermata
Genes referenced: LOC105442066 LOC115919910 LOC373385 LOC581395 LOC587649 mos myod1l


Article Images: [+] show captions
References [+] :
Agrawal, The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1. 2009, Pubmed