ECB-ART-47344Mar Drugs 2019 Jul 25;178:. doi: 10.3390/md17080438.
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Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides.
In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides.
PubMed ID: 31349695
PMC ID: PMC6723998
Article link: Mar Drugs
Genes referenced: eif3d LOC100887844
Article Images: [+] show captions
|Figure 2. (A). Circular dichroism (CD) spectra of ZCPs and ZCP-zinc complexes. (B). Statistical analysis data of CD spectra. (C–E). Molecular weight distribution of ZCPs and ZCP-zinc complexes determined by Raman chromatography (SEC). Values presented are the mean of triplicate analyses.|
|Figure 3. (A). Zeta-potential of ZCPs and ZCP-zinc complexes. (B–D). FTIR spectra of ZCPs and ZCP-zinc complexes. Different letters above the bars indicate significant differences among fractions (p < 0.05). Values presented are the mean of triplicate analyses.|
|Figure 4. (A–C) Raman spectroscopy of ZCPs and ZCP-zinc complexes.|
|Figure 5. (A–C). 13C NMR spectra of ZCPs and ZCP-zinc complexes. (D–F). 1H NMR spectra of ZCPs (with or without D2O) and ZCP-zinc complexes.|
|Figure 6. Schematic model of proposed chelation of sea cucumber-derived peptides with zinc.|
References [+] :
Bozalioğlu, Prevalence of zinc deficiency and immune response in short-term hemodialysis. 2005, Pubmed