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ECB-ART-46879
Biosci Rep 2019 Jan 30;391:. doi: 10.1042/BSR20181312.
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AKT/protein kinase B associates with β-actin in the nucleus of melanoma cells.

Coa LL , Abreu TF , Tashima AK , Green J , Pascon RC , Vallim MA , Machado-Jr J .


Abstract
The serine-threonine kinase AKT/PKB is a critical regulator of various essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. Despite AKT action is known to function mainly in the cytoplasm, AKT has been reported to translocate to the nucleus. However, very little is known about the mechanism required for the nuclear import of AKT as well as its function in this cellular compartment. In the present study, we characterized the presence of endogenous nuclear AKT in human melanoma cells and addressed the possible role of AKT by exploring its potential association with key interaction nuclear partners. Confocal and Western blot analyses showed that both phosphorylated and non-phosphorylated forms of AKT are present in melanoma cells nuclei. Using mass spectrometry in combination with protein-crosslinking and co-immunoprecipitation, we identified a series of putative protein partners of nuclear AKT, including heterogeneous nuclear ribonucleoprotein (hnRNP), cytoskeleton proteins β-actin, γ-actin, β-actin-like 2 and vimentin. Confocal microscopy and biochemical analyses validated β-actin as a new nuclear AKT-interacting partner. Cofilin and active RNA Polymerase II, two proteins that have been described to interact and work in concert with nuclear actin in transcription regulation, were also found associated with nuclear AKT. Overall, the present study uncovered a yet unrecognized nuclear coupling of AKT and provides insights into the involvement of AKT in the interaction network of nuclear actin.

PubMed ID: 30643008
PMC ID: PMC6356016
Article link: Biosci Rep


Genes referenced: btaf1 creb1 ctdp1 LOC115919910 LOC115929578 LOC576396 LOC582192 LOC586660 LOC586734 LOC586799 LOC588766 LOC590297 LOC594261 polr3a tubgcp2


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References [+] :
Adini, RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development. 2003, Pubmed