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Proc Natl Acad Sci U S A
2014 Dec 30;11152:18584-9. doi: 10.1073/pnas.1413282112.
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Allosteric activation of ADAMTS13 by von Willebrand factor.
Muia J
,
Zhu J
,
Gupta G
,
Haberichter SL
,
Friedman KD
,
Feys HB
,
Deforche L
,
Vanhoorelbeke K
,
Westfield LA
,
Roth R
,
Tolia NH
,
Heuser JE
,
Sadler JE
.
Abstract
The metalloprotease ADAMTS13 cleaves von Willebrand factor (VWF) within endovascular platelet aggregates, and ADAMTS13 deficiency causes fatal microvascular thrombosis. The proximal metalloprotease (M), disintegrin-like (D), thrombospondin-1 (T), Cys-rich (C), and spacer (S) domains of ADAMTS13 recognize a cryptic site in VWF that is exposed by tensile force. Another seven T and two complement C1r/C1s, sea urchin epidermal growth factor, and bone morphogenetic protein (CUB) domains of uncertain function are C-terminal to the MDTCS domains. We find that the distal T8-CUB2 domains markedly inhibit substrate cleavage, and binding of VWF or monoclonal antibodies to distal ADAMTS13 domains relieves this autoinhibition. Small angle X-ray scattering data indicate that distal T-CUB domains interact with proximal MDTCS domains. Thus, ADAMTS13 is regulated by substrate-induced allosteric activation, which may optimize VWF cleavage under fluid shear stress in vivo. Distal domains of other ADAMTS proteases may have similar allosteric properties.
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