Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Stress response induced by carbon nanoparticles in Paracentrotus lividus.
Carata E
,
Anna Tenuzzo B
,
Arnò F
,
Buccolieri A
,
Serra A
,
Manno D
,
Dini L
.
???displayArticle.abstract???
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now their toxic effect on development is totally obscure. Embryos treated with carbon nanoparticles, RNA preparation, retro-transcription and quantitative real-time PCR. In response to carbon nano-particles exposure, the embryos collected 24 h later showed a 3,07-fold at 5x10(12) p and a 1,58-fold at 2.5x10(13) p and a 1,92-fold at 2.5x10(14) p increase in Pl14-3-3ε transcript levels compared with controls. The Pl14-3-3ε mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilonisoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3ε. Pl14-3-3ε mRNA levels were measured by RT-PCR during development and found to increase from the mesenchyme blastula to the prism stage. Our results confirm the involvement of 14-3-3ε in the stress response elicited by carbon nano-particles.
Agnello,
Apoptosis: Focus on sea urchin development.
2010, Pubmed,
Echinobase
Agnello,
Apoptosis: Focus on sea urchin development.
2010,
Pubmed
,
Echinobase
Agnello,
Cadmium induces an apoptotic response in sea urchin embryos.
2007,
Pubmed
,
Echinobase
Aitken,
14-3-3 proteins: a historic overview.
2006,
Pubmed
Chaudhri,
Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo.
2003,
Pubmed
Dini,
Phagocytosis of dying cells: influence of smoking and static magnetic fields.
2010,
Pubmed
Dougherty,
Unlocking the code of 14-3-3.
2004,
Pubmed
Fernandez-Guerra,
The genomic repertoire for cell cycle control and DNA metabolism in S. purpuratus.
2006,
Pubmed
,
Echinobase
Garrick,
Parallels and contrasts between iron and copper metabolism.
2003,
Pubmed
Jeanclos,
The chaperone protein 14-3-3eta interacts with the nicotinic acetylcholine receptor alpha 4 subunit. Evidence for a dynamic role in subunit stabilization.
2001,
Pubmed
Kuromori,
Members of the Arabidopsis 14-3-3 gene family trans-complement two types of defects in fission yeast.
2000,
Pubmed
Lau,
Differential role of 14-3-3 family members in Xenopus development.
2006,
Pubmed
Li,
Processable aqueous dispersions of graphene nanosheets.
2008,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Love,
Co-option and dissociation in larval origins and evolution: the sea urchin larval gut.
2008,
Pubmed
,
Echinobase
Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase
Morrison,
The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development.
2009,
Pubmed
Nel,
Toxic potential of materials at the nanolevel.
2006,
Pubmed
Owen,
Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus.
2012,
Pubmed
Paredes,
Graphene oxide dispersions in organic solvents.
2008,
Pubmed
Roberts,
Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants.
1999,
Pubmed
Robertson,
The genomic underpinnings of apoptosis in Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Roccheri,
Cadmium induces the expression of specific stress proteins in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Rosenquist,
Data mining the Arabidopsis genome reveals fifteen 14-3-3 genes. Expression is demonstrated for two out of five novel genes.
2001,
Pubmed
Russo,
Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B.
2010,
Pubmed
,
Echinobase
Russo,
Stress to cadmium monitored by metallothionein gene induction in Paracentrotus lividus embryos.
2003,
Pubmed
,
Echinobase
Samanta,
The transcriptome of the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Tabunoki,
Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60.
2008,
Pubmed
Wang,
Rapid induction of regulatory and transporter genes in response to phosphorus, potassium, and iron deficiencies in tomato roots. Evidence for cross talk and root/rhizosphere-mediated signals.
2002,
Pubmed
