ECB-ART-42494
Mol Reprod Dev
2012 Oct 01;7910:680-8. doi: 10.1002/mrd.22073.
The forkhead transcription factor FoxY regulates Nanos.
Abstract
FoxY is a member of the forkhead transcription factor family that appeared enriched in the presumptive germ line of sea urchins (Ransick et al. Dev Biol 2002;246:132). Here, we test the hypothesis that
FoxY is involved in germ line determination in this animal. We found two splice forms of
FoxY that share the same DNA-binding domain, but vary in the carboxy-terminal trans-activation/repression domain. Both forms of the
FoxY protein are present in the
egg and in the early
embryo , and their mRNAs accumulate to their highest levels in the small
micromeres and adjacent non-skeletogenic
mesoderm . Knockdown of
FoxY resulted in a dramatic decrease in Nanos mRNA and protein levels as well as a loss of coelomic pouches in 2-week-old larvae. Our results indicate that
FoxY positively regulates Nanos at the transcriptional level and is essential for reproductive potential in this organism.
PubMed ID:
22777754
PMC ID:
PMC3473121
Article link:
Mol Reprod Dev
Grant support:
[+]
Genes referenced:
foxy
LOC100887844
Antibodies:
foxy Ab1
foxy Ab2
Morpholinos:
foxy MO1
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