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ECB-ART-42463
Mar Drugs 2012 Apr 01;104:744-761. doi: 10.3390/md10040744.
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Inhibition of hepatitis C virus replication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia.

Yamashita A , Salam KA , Furuta A , Matsuda Y , Fujita O , Tani H , Fujita Y , Fujimoto Y , Ikeda M , Kato N , Sakamoto N , Maekawa S , Enomoto N , Nakakoshi M , Tsubuki M , Sekiguchi Y , Tsuneda S , Akimitsu N , Noda N , Tanaka J , Moriishi K .


Abstract
Hepatitis C virus (HCV) is a causative agent of acute and chronic hepatitis, leading to the development of hepatic cirrhosis and hepatocellular carcinoma. We prepared extracts from 61 marine organisms and screened them by an in vitro fluorescence assay targeting the viral helicase (NS3), which plays an important role in HCV replication, to identify effective candidates for anti-HCV agents. An ethyl acetate-soluble fraction of the feather star Alloeocomatella polycladia exhibited the strongest inhibition of NS3 helicase activity, with an IC(50) of 11.7 µg/mL. The extract of A. polycladia inhibited interaction between NS3 and RNA but not ATPase of NS3. Furthermore, the replication of the replicons derived from three HCV strains of genotype 1b in cultured cells was suppressed by the extract with an EC(50) value of 23 to 44 µg/mL, which is similar to the IC(50) value of the NS3 helicase assay. The extract did not induce interferon or inhibit cell growth. These results suggest that the unknown compound(s) included in A. polycladia can inhibit HCV replication by suppressing the helicase activity of HCV NS3. This study may present a new approach toward the development of a novel therapy for chronic hepatitis C.

PubMed ID: 22690141
PMC ID: PMC3366673
Article link: Mar Drugs


Genes referenced: LOC100893907 LOC115919910 LOC579132 LOC581395 LOC587333 LOC587482 LOC587800 LOC590297 polr3a


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References [+] :
Ahmed-Belkacem, Silibinin and related compounds are direct inhibitors of hepatitis C virus RNA-dependent RNA polymerase. 2010, Pubmed