Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Cell Stress Chaperones
2010 Nov 01;156:993-1001. doi: 10.1007/s12192-010-0210-1.
Show Gene links
Show Anatomy links
Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B.
Russo R
,
Zito F
,
Costa C
,
Bonaventura R
,
Matranga V
.
???displayArticle.abstract???
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilon isoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3ε, and the phylogenetic relationship with other homologues described in different phyla. Pl14-3-3ε mRNA levels were measured by QPCR during development and found to increase from the mesenchyme blastula to the prism stage. In response to UV-B (312 nm) exposure, early stage embryos collected 2 h later showed a 2.3-fold (at 400 J/m(2)) and a 2.7-fold (at 800 J/m(2)) increase in Pl14-3-3ε transcript levels compared with controls. The spatial expression of Pl14-3-3ε mRNA, detected by whole mount in situ hybridization in both control and UV-B exposed embryos, harvested at late developmental stages, showed transcripts to be located in the archenteron of gastrula stage and widely distributed in all germ layers, respectively. The Pl14-3-3ε mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers, investigated by indirect immuno-fluorescence on whole mount embryos. Results confirm the involvement of 14-3-3ε in the stress response elicited by UV-B and demonstrate, for the first time, its contribution at the transcriptional level in the sea urchin embryo.
Acevedo,
In vivo functional specificity and homeostasis of Drosophila 14-3-3 proteins.
2007, Pubmed
Acevedo,
In vivo functional specificity and homeostasis of Drosophila 14-3-3 proteins.
2007,
Pubmed
Agnello,
Apoptosis: Focus on sea urchin development.
2010,
Pubmed
,
Echinobase
Agnello,
Cadmium induces an apoptotic response in sea urchin embryos.
2007,
Pubmed
,
Echinobase
Aitken,
14-3-3 proteins: a historic overview.
2006,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Autier,
Melanoma and use of sunscreens: an Eortc case-control study in Germany, Belgium and France. The EORTC Melanoma Cooperative Group.
1995,
Pubmed
Banaszak,
Effects of solar ultraviolet radiation on coral reef organisms.
2009,
Pubmed
Batel,
Expression of the human XPB/ERCC-3 excision repair gene-homolog in the sponge Geodia cydonium after exposure to ultraviolet radiation.
1998,
Pubmed
Bonaventura,
UVB radiation prevents skeleton growth and stimulates the expression of stress markers in sea urchin embryos.
2005,
Pubmed
,
Echinobase
Chaudhri,
Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo.
2003,
Pubmed
Choi,
Increased expression of 14-3-3varepsilon protein in intrinsically aged and photoaged human skin in vivo.
2005,
Pubmed
Diffey,
Sources and measurement of ultraviolet radiation.
2002,
Pubmed
Dougherty,
Unlocking the code of 14-3-3.
2004,
Pubmed
Duboc,
Nodal and BMP2/4 pattern the mesoderm and endoderm during development of the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Fernandez-Guerra,
The genomic repertoire for cell cycle control and DNA metabolism in S. purpuratus.
2006,
Pubmed
,
Echinobase
Gerhard,
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
2004,
Pubmed
Gies,
Global Solar UV Index: Australian measurements, forecasts and comparison with the UK.
2004,
Pubmed
Hader,
Effects of solar UV-B radiation on aquatic ecosystems.
2000,
Pubmed
Hanson,
Sunscreen enhancement of UV-induced reactive oxygen species in the skin.
2006,
Pubmed
Holick,
Environmental factors that influence the cutaneous production of vitamin D.
1995,
Pubmed
Holzinger,
Algae and UV irradiation: effects on ultrastructure and related metabolic functions.
2006,
Pubmed
Jeanclos,
The chaperone protein 14-3-3eta interacts with the nicotinic acetylcholine receptor alpha 4 subunit. Evidence for a dynamic role in subunit stabilization.
2001,
Pubmed
Kiyomoto,
Skeletogenesis by transfated secondary mesenchyme cells is dependent on extracellular matrix-ectoderm interactions in Paracentrotus lividus sea urchin embryos.
2007,
Pubmed
,
Echinobase
Kuromori,
Members of the Arabidopsis 14-3-3 gene family trans-complement two types of defects in fission yeast.
2000,
Pubmed
Lau,
Differential role of 14-3-3 family members in Xenopus development.
2006,
Pubmed
Leffers,
Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway.
1993,
Pubmed
Lesser,
Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos.
2003,
Pubmed
,
Echinobase
Lesser,
Depth-dependent effects of ultraviolet radiation on survivorship, oxidative stress and DNA damage in sea urchin (Strongylocentrotus droebachiensis) embryos from the Gulf of Maine.
2010,
Pubmed
,
Echinobase
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Loser,
Regulation of cutaneous immunity by the environment: an important role for UV irradiation and vitamin D.
2009,
Pubmed
Love,
Co-option and dissociation in larval origins and evolution: the sea urchin larval gut.
2008,
Pubmed
,
Echinobase
Mackintosh,
Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes.
2004,
Pubmed
Matsumura,
Toxic effects of ultraviolet radiation on the skin.
2004,
Pubmed
Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase
Morrison,
The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development.
2009,
Pubmed
Perego,
Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line.
1997,
Pubmed
Petrocelli,
UVB induced cell cycle checkpoints in an early stage human melanoma line, WM35.
2000,
Pubmed
Roberts,
Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants.
1999,
Pubmed
Robertson,
The genomic underpinnings of apoptosis in Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Roccheri,
Cadmium induces the expression of specific stress proteins in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Rosenquist,
Data mining the Arabidopsis genome reveals fifteen 14-3-3 genes. Expression is demonstrated for two out of five novel genes.
2001,
Pubmed
Russo,
Stress to cadmium monitored by metallothionein gene induction in Paracentrotus lividus embryos.
2003,
Pubmed
,
Echinobase
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Samanta,
The transcriptome of the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Schröder,
DNA damage and developmental defects after exposure to UV and heavy metals in sea urchin cells and embryos compared to other invertebrates.
2005,
Pubmed
,
Echinobase
Smith,
Ozone depletion: ultraviolet radiation and phytoplankton biology in antarctic waters.
1992,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Tabunoki,
Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60.
2008,
Pubmed
Tedetti,
Penetration of ultraviolet radiation in the marine environment. A review.
2006,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Wang,
Molecular evolution of the 14-3-3 protein family.
1996,
Pubmed
Wessel,
Sequential expression of germ-layer specific molecules in the sea urchin embryo.
1985,
Pubmed
,
Echinobase
de la Fuente,
Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells.
2009,
Pubmed
