Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network.
Henson JH
,
Cheung D
,
Fried CA
,
Shuster CB
,
McClellan MK
,
Voss MK
,
Sheridan JT
,
Oldenbourg R
.
Abstract
Sea urchin coelomocytes contain an unusually broad lamellipodial region and have served as a useful model experimental system for studying the process of actin-based retrograde/centripetal flow. In the current study the small molecule drug 2,3-butanedione monoxime (BDM) was employed as a means of delocalizing the Arp2/3 complex from the cell edge in an effort to investigate the Arp2/3 complex-independent aspects of retrograde flow. Digitally-enhanced phase contrast, fluorescence and polarization light microscopy, along with rotary shadow transmission electron microscopy methods demonstrated that BDM treatment resulted in the centripetal displacement of the Arp2/3 complex and the associated dendritic lamellipodial (LP) actin network from the cell edge. In its wake there remained an array of elongate actin filaments organized into concave arcs that displayed retrograde flow at approximately one quarter the normal rate. Actin polymerization inhibitor experiments indicated that these arcs were generated by polymerization at the cell edge, while active myosin-based contraction in BDM treated cells was demonstrated by localization with antiphospho-myosin regulatory light chain (MRLC) antibody, the retraction of the cytoskeleton in the presence of BDM, and the response of the BDM arcs to laser-based severing. The results suggest that BDM treatment reveals an Arp2/3 complex-independent actin structure in coelomocytes consisting of elongate filaments integrated into the LP network and that these filaments represent a potential connection between the LP network and the central cytoskeleton.
Bernstein,
Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF).
1982, Pubmed
Bernstein,
Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF).
1982,
Pubmed
Blanchoin,
Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin.
2001,
Pubmed
Broschat,
Tropomyosin prevents depolymerization of actin filaments from the pointed end.
1990,
Pubmed
Cheung,
A small-molecule inhibitor of skeletal muscle myosin II.
2002,
Pubmed
Chhabra,
The many faces of actin: matching assembly factors with cellular structures.
2007,
Pubmed
Colombelli,
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery.
2005,
Pubmed
Cramer,
Myosin is involved in postmitotic cell spreading.
1995,
Pubmed
Danilchik,
Intrinsic chiral properties of the Xenopus egg cortex: an early indicator of left-right asymmetry?
2006,
Pubmed
Danuser,
Coupling the dynamics of two actin networks--new views on the mechanics of cell protrusion.
2005,
Pubmed
DesMarais,
Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
2002,
Pubmed
Durán,
Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport.
2003,
Pubmed
Edds,
Isolation and characterization of two forms of a cytoskeleton.
1979,
Pubmed
,
Echinobase
Esseling-Ozdoba,
Hydrodynamic flow in the cytoplasm of plant cells.
2008,
Pubmed
Evangelista,
Formins: signaling effectors for assembly and polarization of actin filaments.
2003,
Pubmed
Faix,
Staying in shape with formins.
2006,
Pubmed
Forer,
Does 2,3-butanedione monoxime inhibit nonmuscle myosin?
2005,
Pubmed
Forscher,
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
1988,
Pubmed
Fryer,
Effects of 2,3-butanedione monoxime on the contractile activation properties of fast- and slow-twitch rat muscle fibres.
1988,
Pubmed
Funaki,
The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM).
2004,
Pubmed
Giannone,
Periodic lamellipodial contractions correlate with rearward actin waves.
2004,
Pubmed
Gupton,
mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration.
2007,
Pubmed
Gupton,
Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin.
2005,
Pubmed
Gwathmey,
Contractile deactivation and uncoupling of crossbridges. Effects of 2,3-butanedione monoxime on mammalian myocardium.
1991,
Pubmed
Henson,
Two components of actin-based retrograde flow in sea urchin coelomocytes.
1999,
Pubmed
,
Echinobase
Henson,
Calcium regulation of the actin-mediated cytoskeletal transformation of sea urchin coelomocytes.
1983,
Pubmed
,
Echinobase
Henson,
Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
1992,
Pubmed
,
Echinobase
Henson,
Actin-based centripetal flow: phosphatase inhibition by calyculin-A alters flow pattern, actin organization, and actomyosin distribution.
2003,
Pubmed
,
Echinobase
Henson,
Wound closure in the lamellipodia of single cells: mediation by actin polymerization in the absence of an actomyosin purse string.
2002,
Pubmed
,
Echinobase
Iwasa,
Spatial and temporal relationships between actin-filament nucleation, capping, and disassembly.
2007,
Pubmed
Koestler,
Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front.
2008,
Pubmed
Kumar,
Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics.
2006,
Pubmed
LeClaire,
Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments.
2008,
Pubmed
Lee,
Actin dysfunction activates ERK1/2 and delays entry into mitosis in mammalian cells.
2007,
Pubmed
Lu,
Actin stress fiber pre-extension in human aortic endothelial cells.
2008,
Pubmed
McCurdy,
Is 2,3-butanedione monoxime an effective inhibitor of myosin-based activities in plant cells?
1999,
Pubmed
Medeiros,
Myosin II functions in actin-bundle turnover in neuronal growth cones.
2006,
Pubmed
Nemethova,
Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.
2008,
Pubmed
Ostap,
2,3-Butanedione monoxime (BDM) as a myosin inhibitor.
2002,
Pubmed
Osterman,
Effects of 2,3-butanedione monoxime on activation of contraction and crossbridge kinetics in intact and chemically skinned smooth muscle fibres from guinea pig taenia coli.
1993,
Pubmed
Otto,
Formation of filopodia in coelomocytes: localization of fascin, a 58,000 dalton actin cross-linking protein.
1979,
Pubmed
,
Echinobase
Pollard,
Regulation of actin filament assembly by Arp2/3 complex and formins.
2007,
Pubmed
Pollard,
Cellular motility driven by assembly and disassembly of actin filaments.
2003,
Pubmed
Ponti,
Two distinct actin networks drive the protrusion of migrating cells.
2004,
Pubmed
Rogers,
Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
2003,
Pubmed
Sambrano,
Navigating the signalling network in mouse cardiac myocytes.
2002,
Pubmed
Shaner,
Actin and alpha-actinin dynamics in the adhesion and motility of EPEC and EHEC on host cells.
2005,
Pubmed
Small,
Actin filament organization in the fish keratocyte lamellipodium.
1995,
Pubmed
Small,
Unravelling the structure of the lamellipodium.
2008,
Pubmed
Svitkina,
Electron microscopic analysis of the leading edge in migrating cells.
2007,
Pubmed
Svitkina,
Mechanism of filopodia initiation by reorganization of a dendritic network.
2003,
Pubmed
Svitkina,
Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia.
1999,
Pubmed
Thum,
Reprogramming of gene expression in cultured cardiomyocytes and in explanted hearts by the myosin ATPase inhibitor butanedione monoxime.
2001,
Pubmed
Thum,
Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary cultures.
2001,
Pubmed
Turvey,
Modulation of IP(3)-sensitive Ca(2+) release by 2,3-butanedione monoxime.
2003,
Pubmed
Vallotton,
Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy.
2004,
Pubmed
Verkhovsky,
Orientational order of the lamellipodial actin network as demonstrated in living motile cells.
2003,
Pubmed
Warnecke,
Improved right heart function after myocardial preservation with 2,3-butanedione 2-monoxime in a porcine model of allogenic heart transplantation.
2002,
Pubmed
Watanabe,
Inhibitory effect of 2,3-butanedione monoxime (BDM) on Na(+)/Ca(2+) exchange current in guinea-pig cardiac ventricular myocytes.
2001,
Pubmed
Yang,
Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells.
2007,
Pubmed
Yarrow,
Rapid de-localization of actin leading edge components with BDM treatment.
2003,
Pubmed
Zhang,
Rho-dependent contractile responses in the neuronal growth cone are independent of classical peripheral retrograde actin flow.
2003,
Pubmed
Zhou,
Myosin II is present in gastric parietal cells and required for lamellipodial dynamics associated with cell activation.
2003,
Pubmed
