ECB-ART-39822Mar Environ Res 2006 Jul 01;62 Suppl:S1-4. doi: 10.1016/j.marenvres.2006.04.062.
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The sea urchin embryo as a model for studying efflux transporters: roles and energy cost.
We describe the use of the sea urchin as a model for studying efflux transporters and estimating energy cost for the cytotoxin protective system provided by these transporters. The unfertilized egg has low transport activity, which increases to a new steady state shortly after fertilization. Activity results from p-glycoprotein (p-gp) and MRP type transporters which protect the embryo from cytotoxic drugs that can disrupt cell division or induce apoptosis. The energy cost is estimated from a novel use of calcein-AM as a substrate; keeping 0.25 microM substrate levels out of the cell utilizes only 0.023% of steady state respiration.
PubMed ID: 16740304
PMC ID: PMC3160780
Article link: Mar Environ Res
Genes referenced: LOC100887844 LOC115919910 LOC582726
References [+] :
Essodaigui, Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein. 1998, Pubmed