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Biophys J 2005 Jun 01;886:L46-8. doi: 10.1529/biophysj.105.063768.
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Second harmonic imaging of exocytosis at fertilization.

Millard AC , Terasaki M , Loew LM .

Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time.

PubMed ID: 15849238
PMC ID: PMC1305673
Article link: Biophys J
Grant support: [+]

Genes referenced: LOC100887844 LOC115925415

References [+] :
Berg, Cortical granules of the sea urchin translocate early in oocyte maturation. 1997, Pubmed, Echinobase