ECB-ART-39138Dev Biol 2004 Sep 15;2732:226-43. doi: 10.1016/j.ydbio.2004.05.033.
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SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis.
The Strongylocentrotus purpuratus hnf6 (Sphnf6) gene encodes a new member of the ONECUT family of transcription factors. The expression of hnf6 in the developing embryo is triphasic, and loss-of-function analysis shows that the Hnf6 protein is a transcription factor that has multiple distinct roles in sea urchin development. hnf6 is expressed maternally, and before gastrulation its transcripts are distributed globally. Early in development, its expression is required for the activation of PMC differentiation genes such as sm50, pm27, and msp130, but not for the activation of any known PMC regulatory genes, for example, alx, ets1, pmar1, or tbrain. Micromere transplantation experiments show that the gene is not involved in early micromere signaling. Early hnf6 expression is also required for expression of the mesodermal regulator gatac. The second known role of hnf6 is its participation after gastrulation in the oral ectoderm gene regulatory network (GRN), in which its expression is essential for the maintenance of the state of oral ectoderm specification. The third role is in the neurogenic ciliated band, which is foreshadowed exactly by a trapezoidal band of hnf6 expression at the border of the oral ectoderm and where it continues to be expressed through the end of embryogenesis. Neither oral ectoderm regulatory functions nor ciliated band formation occur normally in the absence of hnf6 expression.
PubMed ID: 15328009
Article link: Dev Biol
Genes referenced: ets1 gata3 hnf6 LOC100887844 LOC115924597 LOC575170 msp130 onecut2 pm27 pmar1
Antibodies: ctnnb1 Ab1 onecut2 Ab1 sox2 Ab1
Morpholinos: onecut2 MO1