ECB-ART-37458Dev Biol 2000 Jun 15;2222:420-8. doi: 10.1006/dbio.2000.9726.
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MAP kinase, a universal suppressor of sperm centrosomes during meiosis?
We reported previously that inhibition of MAP kinase during meiosis in Urechis caupo eggs caused premature sperm aster formation and we reviewed indirect evidence that the suppression of sperm asters by MAPK during meiosis might be a universal mechanism (M. C. Gould and J. L. Stephano, 1999, Dev. Biol. 216, 348-358). We tested this proposition with oyster (Crassostrea gigas) and starfish (Asterina miniata) eggs, utilizing the MEK inhibitors U0126 and PD98059. Centrosomes, asters, and meiotic spindles were visualized by normal epifluorescence and confocal microscopy following indirect immunocytochemical staining for anti-beta-tubulin. When MAPK activation was inhibited, sperm asters in both species developed prematurely and tended to move toward the egg centrosomes, sometimes even fusing with the egg spindle or centrosomes. Meiotic spindles and polar body formation were also abnormal when MAPK was inhibited.
PubMed ID: 10837129
Article link: Dev Biol
Genes referenced: LOC576066 tubgcp2
Antibodies: phospho-mapk1 Ab3