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Dev Genes Evol 2000 Feb 01;2102:73-81. doi: 10.1007/s004270050013.
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Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins.

Huang L , Li X , El-Hodiri HM , Dayal S , Wikramanayake AH , Klein WH .

Members of the Tcf/Lef family interact with beta-catenin to activate programs of gene expression during development. Recently beta-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when beta-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with beta-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of beta-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with beta-catenin to specify cell fates along the sea urchin animal-vegetal axis.

PubMed ID: 10664150
Article link: Dev Genes Evol
Grant support: [+]

Genes referenced: LOC100887844 LOC115919910 LOC115921693 LOC594353 Tcf/Lef