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Stimulation of Xenopus oocyte maturation by inhibition of the G-protein alpha S subunit, a component of the plasma membrane and yolk platelet membranes.
Abstract
Oocytes of Xenopus laevis undergo maturation when injected with an affinity-purified antibody against the COOH-terminal decapeptide of the alpha subunit of the G-protein Gs, an antibody that inhibits Gs activity. Germinal vesicle breakdown, chromosome condensation, and polar body formation occur, with a time course similar to that for oocytes treated with progesterone. The alpha S antibody-injected oocytes also acquire the ability to be activated by sperm. Coinjection of the catalytic subunit of cAMP-dependent protein kinase, or incubation with cycloheximide, inhibits maturation in response to injection of the alpha S antibody; these experiments show that the alpha S antibody acts at an early point in the pathway leading to oocyte maturation, before formation of maturation promoting factor, and like progesterone, its action requires protein synthesis. Immunogold electron microscopy shows that alpha S is present in the yolk platelet membranes as well as the plasma membrane. These results support the hypothesis that progesterone acts by inhibiting alpha S, and suggest that the target of progesterone could include yolk platelet membranes as well as the plasma membrane.
Antonelli,
Human-Xenopus chimeras of Gs alpha reveal a new region important for its activation of adenylyl cyclase.
1994, Pubmed
Antonelli,
Human-Xenopus chimeras of Gs alpha reveal a new region important for its activation of adenylyl cyclase.
1994,
Pubmed
Aridor,
Activation of exocytosis by the heterotrimeric G protein Gi3.
1993,
Pubmed
Baldwin,
Structure and function of receptors coupled to G proteins.
1994,
Pubmed
Bomsel,
Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor.
1993,
Pubmed
Bomsel,
Role of heterotrimeric G proteins in membrane traffic.
1992,
Pubmed
Bronson,
Autoradiography of progesterone and model compound entry and distribution in Xenopus laevis oocytes.
1991,
Pubmed
Chen,
Suppression of Ras-induced transformation of NIH 3T3 cells by activated G alpha s.
1994,
Pubmed
Chiba,
The primary structure of the alpha subunit of a starfish guanosine-nucleotide-binding regulatory protein involved in 1-methyladenine-induced oocyte maturation.
1992,
Pubmed
,
Echinobase
Chiba,
Induction of starfish oocyte maturation by the beta gamma subunit of starfish G protein and possible existence of the subsequent effector in cytoplasm.
1993,
Pubmed
,
Echinobase
Cicirelli,
Cyclic AMP levels during the maturation of Xenopus oocytes.
1985,
Pubmed
Colombo,
Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion.
1992,
Pubmed
Colombo,
Gs regulation of endosome fusion suggests a role for signal transduction pathways in endocytosis.
1994,
Pubmed
Conklin,
Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.
1993,
Pubmed
Cork,
Microinjected GTP-gamma-S inhibits progesterone-induced maturation of Xenopus oocytes.
1990,
Pubmed
Daar,
Inhibition of mos-induced oocyte maturation by protein kinase A.
1993,
Pubmed
Danilchik,
Differentiation of the animal-vegetal axis in Xenopus laevis oocytes. I. Polarized intracellular translocation of platelets establishes the yolk gradient.
1987,
Pubmed
Dascal,
Further characterization of the slow muscarinic responses in Xenopus oocytes.
1987,
Pubmed
Duesbery,
Changes in protein association with intracellular membranes of Xenopus laevis oocytes during maturation and activation.
1993,
Pubmed
Elinson,
Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs.
1993,
Pubmed
Fagotto,
Yolk platelets in Xenopus oocytes maintain an acidic internal pH which may be essential for sodium accumulation.
1994,
Pubmed
Finidori-Lepicard,
Progesterone inhibits membrane-bound adenylate cyclase in Xenopus laevis oocytes.
1981,
Pubmed
Gard,
Microtubule organization during maturation of Xenopus oocytes: assembly and rotation of the meiotic spindles.
1992,
Pubmed
Gard,
Organization, nucleation, and acetylation of microtubules in Xenopus laevis oocytes: a study by confocal immunofluorescence microscopy.
1991,
Pubmed
Godeau,
Induction of maturation in Xenopus laevis oocytes by a steroid linked to a polymer.
1978,
Pubmed
Goodhardt,
Does the guanine nucleotide regulatory protein Ni mediate progesterone inhibition of Xenopus oocyte adenylate cyclase?
1984,
Pubmed
Hepler,
G proteins.
1992,
Pubmed
Hiramoto,
A method of microinjection.
1974,
Pubmed
Huchon,
The pure inhibitor of cAMP-dependent protein kinase initiates Xenopus laevis meiotic maturation. A 4-step scheme for meiotic maturation.
1981,
Pubmed
Ishikawa,
Primary action of steroid hormone at the surface of amphibian oocyte in the induction of germinal vesicle breakdown.
1977,
Pubmed
Jaffe,
Oocyte maturation in starfish is mediated by the beta gamma-subunit complex of a G-protein.
1993,
Pubmed
,
Echinobase
Jordana,
Studies on the mechanism of inhibition of amphibian oocyte adenylate cyclase by progesterone.
1984,
Pubmed
Kline,
Evidence for the involvement of a pertussis toxin-insensitive G-protein in egg activation of the frog, Xenopus laevis.
1991,
Pubmed
Kroll,
Activated alpha subunit of Go protein induces oocyte maturation.
1991,
Pubmed
Liu,
High-affinity binding of progesterone to the plasma membrane of Xenopus oocytes: characteristics of binding and hormonal and developmental control.
1993,
Pubmed
Maller,
Early effect of progesterone on levels of cyclic adenosine 3':5'-monophosphate in Xenopus oocytes.
1979,
Pubmed
Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
Mallya,
Proteolysis of the major yolk glycoproteins is regulated by acidification of the yolk platelets in sea urchin embryos.
1992,
Pubmed
,
Echinobase
Masui,
Towards understanding the control of the division cycle in animal cells.
1992,
Pubmed
Masui,
Oocyte maturation.
1979,
Pubmed
Meinkoth,
Inhibition of thyrotropin-induced DNA synthesis in thyroid follicular cells by microinjection of an antibody to the stimulatory G protein of adenylate cyclase, Gs.
1992,
Pubmed
Milano,
Enhanced myocardial function in transgenic mice overexpressing the beta 2-adrenergic receptor.
1994,
Pubmed
Miledi,
Effects of defolliculation on membrane current responses of Xenopus oocytes.
1989,
Pubmed
Mulner,
Pertussis toxin facilitates the progesterone-induced maturation of Xenopus oocyte. Possible role of protein phosphorylation.
1985,
Pubmed
Nair,
Gs alpha mediates epidermal growth factor-elicited stimulation of rat cardiac adenylate cyclase.
1990,
Pubmed
Negishi,
Opposite coupling of prostaglandin E receptor EP3C with Gs and G(o). Stimulation of Gs and inhibition of G(o).
1993,
Pubmed
Noel,
The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S.
1993,
Pubmed
Olate,
Molecular cloning and sequence determination of four different cDNA species coding for alpha-subunits of G proteins from Xenopus laevis oocytes.
1990,
Pubmed
Olate,
Oocyte adenylyl cyclase contains Ni, yet the guanine nucleotide-dependent inhibition by progesterone is not sensitive to pertussis toxin.
1984,
Pubmed
OƱate,
Xenopus laevis oocyte G alpha subunits mRNAs. Detection and quantitation during oogenesis and early embryogenesis by competitive reverse PCR.
1992,
Pubmed
Pellaz,
Evidence for a pertussis toxin-sensitive G protein involved in the control of meiotic reinitiation of Xenopus laevis oocytes.
1989,
Pubmed
Pimplikar,
Regulation of apical transport in epithelial cells by a Gs class of heterotrimeric G protein.
1993,
Pubmed
Roychowdhury,
Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction.
1994,
Pubmed
Roychowdhury,
G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements.
1993,
Pubmed
Sadler,
Inhibition of Xenopus oocyte adenylate cyclase by progesterone: a novel mechanism of action.
1985,
Pubmed
Sadler,
Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.
1981,
Pubmed
Sadler,
Progesterone inhibition of Xenopus oocyte adenylate cyclase is not mediated via the Bordetella pertussis toxin substrate.
1984,
Pubmed
Sadler,
Inhibition of Xenopus oocyte adenylate cyclase by progesterone and 2',5'-dideoxyadenosine is associated with slowing of guanine nucleotide exchange.
1983,
Pubmed
Salomon,
A highly sensitive adenylate cyclase assay.
1974,
Pubmed
Shilling,
Pertussis toxin inhibits 1-methyladenine-induced maturation in starfish oocytes.
1989,
Pubmed
,
Echinobase
Simonds,
Receptor and effector interactions of Gs. Functional studies with antibodies to the alpha s carboxyl-terminal decapeptide.
1989,
Pubmed
Simonds,
Gi2 mediates alpha 2-adrenergic inhibition of adenylyl cyclase in platelet membranes: in situ identification with G alpha C-terminal antibodies.
1989,
Pubmed
Tadenuma,
Properties of 1-methyladenine receptors in starfish oocyte membranes: involvement of pertussis toxin-sensitive GTP-binding protein in the receptor-mediated signal transduction.
1992,
Pubmed
,
Echinobase
Tadenuma,
Purification and characterization of a GTP-binding protein serving as pertussis toxin substrate in starfish oocytes.
1991,
Pubmed
,
Echinobase
Thambi,
Immunological and molecular characterization of Go alpha-like proteins in the Drosophila central nervous system.
1989,
Pubmed
Tilley,
The jelly layers and cortex of the unfertilized Xenopus laevis egg: carbohydrate, phospholipid and protein analysis.
1988,
Pubmed
Tso,
Microinjected progesterone reinitiates meiotic maturation of Xenopus laevis oocytes.
1982,
Pubmed
Wasserman,
Effects of cyclohexamide on a cytoplasmic factor initiating meiotic naturation in Xenopus oocytes.
1975,
Pubmed
Wilson,
Endoplasmic reticulum-through-Golgi transport assay based on O-glycosylation of native glycophorin in permeabilized erythroleukemia cells: role for Gi3.
1993,
Pubmed
Yoshikuni,
Characterization of 1-methyladenine binding in starfish oocyte cortices.
1988,
Pubmed
,
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