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Mol Biol Cell
1995 Jun 01;66:685-96. doi: 10.1091/mbc.6.6.685.
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Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses.
Ogawa K
,
Kamiya R
,
Wilkerson CG
,
Witman GB
.
Abstract
Immunological analysis showed that antibodies against the intermediate chains (ICs) IC2 and IC3 of sea urchin outer arm dynein specifically cross-reacted with intermediate chains IC78 and IC69, respectively, of Chlamydomonas outer arm dynein. In contrast, no specific cross-reactivity with any Chlamydomonas outer arm polypeptide was observed using antibody against IC1 of sea urchin outer arm dynein. To learn more about the relationships between the different ICs, overlapping cDNAs encoding all of IC2 and IC3 of sea urchin were isolated and sequenced. Comparison of these sequences with those previously obtained for the Chlamydomonas ICs revealed that, although all four chains are homologous, sea urchin IC2 is much more closely related to Chlamydomonas IC78 (45.8% identity), and sea urchin IC3 is much more closely related to Chlamydomonas IC69 (48.5% identity), than either sea urchin chain is related to the other (23.5% identity). For homologous pairs, the similarities extend throughout the full lengths of the chains. Regions of similarity between all four ICs and the IC (IC74) of cytoplasmic dynein, located in the C-terminal halves of the chains, are due primarily to conservation of the WD repeats present in all of these ICs. This is the first demonstration that structural differences between individual ICs within an outer arm dynein have been highly conserved in the dyneins of distantly related species. The results provide a basis for the subclassification of these chains.
Bell,
Polypeptide subunits of dynein 1 from sea urchin sperm flagella.
1979, Pubmed,
Echinobase
Bell,
Polypeptide subunits of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Gagnon,
A monoclonal antibody against the dynein IC1 peptide of sea urchin spermatozoa inhibits the motility of sea urchin, dinoflagellate, and human flagellar axonemes.
1994,
Pubmed
,
Echinobase
Gatti,
Outer arm dynein from trout spermatozoa. Purification, polypeptide composition, and enzymatic properties.
1989,
Pubmed
Higgins,
CLUSTAL V: multiple alignment of DNA and protein sequences.
1994,
Pubmed
Kamiya,
Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii.
1988,
Pubmed
King,
Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy.
1990,
Pubmed
King,
The Mr 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with alpha-tubulin in situ.
1991,
Pubmed
King,
Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting.
1985,
Pubmed
,
Echinobase
King,
Outer-arm dynein from trout spermatozoa: substructural organization.
1990,
Pubmed
Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed
Mierendorf,
Gene isolation by screening lambda gt11 libraries with antibodies.
1987,
Pubmed
Mitchell,
Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene.
1991,
Pubmed
Mitchell,
Reversion analysis of dynein intermediate chain function.
1993,
Pubmed
Mitchell,
Protein-protein interactions in the 18S ATPase of Chlamydomonas outer dynein arms.
1986,
Pubmed
Moss,
The alpha subunit of sea urchin sperm outer arm dynein mediates structural and rigor binding to microtubules.
1992,
Pubmed
,
Echinobase
Neer,
The ancient regulatory-protein family of WD-repeat proteins.
1994,
Pubmed
Paschal,
Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function.
1992,
Pubmed
Porter,
Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms.
1992,
Pubmed
Porter,
Characterization of the ATP-sensitive binding of Tetrahymena 30 S dynein to bovine brain microtubules.
1983,
Pubmed
Sale,
The substructure of isolated and in situ outer dynein arms of sea urchin sperm flagella.
1985,
Pubmed
,
Echinobase
Sale,
Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.
1988,
Pubmed
,
Echinobase
Smith,
Microtubule binding and translocation by inner dynein arm subtype I1.
1991,
Pubmed
Tang,
Structure of the dynein-1 outer arm in sea urchin sperm flagella. I. Analysis by separation of subunits.
1982,
Pubmed
,
Echinobase
Wilkerson,
The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly.
1995,
Pubmed
Witman,
Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.
1978,
Pubmed
van der Voorn,
The WD-40 repeat.
1992,
Pubmed