Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Biochem J 1968 Apr 01;1073:403-10. doi: 10.1042/bj1070403.
Show Gene links Show Anatomy links

Variations in the phosphate content and thiołdisulphide ratio of histones during the cell cycle. Studies with regenerating rat liver and sea urchins.

Ord MG , Stocken LA .

1. In regenerating rat liver the phosphate content of the lysine-rich histone F1, but not that of the more arginine-rich histone F3-1, increases during the period of DNA synthesis. 2. The phosphorylation of histone F1 in this ;S period'' is decreased by gamma-irradiation, but, like phosphate uptake into DNA, is affected to an even greater extent if the irradiation is given in the presynthetic period. 3. Histones from three species of sea-urchin eggs show similarities to the F2 and F3 groups of histones from mammalian thymus gland. 4. The proportion of thiol to total thiol plus disulphide in acid extracts from sea-urchin eggs varies from less than 20% in mature unfertilized eggs to 59% just before cleavage. 5. The phosphorylated forms of histones F1 and F3 are less effective in decreasing DNA synthesis by DNA polymerase than the non-phosphorylated forms. 6. Oxidation of thiol groups on histone F3-1 does not affect its capacity to decrease DNA synthesis in vitro.

PubMed ID: 5689839
PMC ID: PMC1198677
Article link: Biochem J

Genes referenced: LOC100887844 LOC115919910 LOC115923832 LOC579470 polr3a

References [+] :
BARTLETT, Phosphorus assay in column chromatography. 1959, Pubmed