ECB-ART-33952Biochem J 1970 Mar 01;1171:57-9. doi: 10.1042/bj1170057.
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Zymogen activation as a sensitive enzyme-amplifying assay for a protease with tryptic specificity.
A protease, ;trypsinogenase'', secreted in small amounts by the sea-urchin blastula, is assayed in two steps as an example of enzyme-amplifying kinetics. In reaction 1 the trypsinogenase catalyses the activation of trypsinogen to trypsin. In reaction 2 the trypsin catalyses the hydrolysis of N-alpha-toluene-p-sulphonylarginine methyl ester, at a rate that is linear with trypsinogenase concentration over a 20-fold range. Results are reproducible within a batch of zymogen, but each batch requires a separate standard curve.
PubMed ID: 5420957
PMC ID: PMC1178829
Article link: Biochem J
Genes referenced: LOC100887844 LOC752081 LOC756768
References [+] :
Barrett, Maternal characteristics of hatching enzymes in hybrid sea urchin embryos. 1969, Pubmed, Echinobase