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Dev Biol 1987 Nov 01;1241:281-6. doi: 10.1016/0012-1606(87)90479-9.
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Changes in the catalytic properties of DNA ligases during early sea urchin development.

Prigent C , Maniey D , Lefresne J , Epel D , Signoret J , David JC .

Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertilization. After the two proteins were purified, their catalytic properties were studied using different substrates. The oocyte ligase acts only on deoxypolymers while the embryonic form also ligates heteropolymers. The two enzymes were found to undergo both nick and cohesive-end ligation. With different kinds of restriction sites it was observed that the embryonic enzyme could also ligate blunt-ended DNA. These catalytic properties account for sealing of exogenous DNA and concatenation following DNA injection into eggs. The role of the oocyte form of the enzyme is unclear; one speculation is a role in repair of DNA breaks which might accumulate during long-term sperm and oocyte storage in the gonad.

PubMed ID: 3666310
Article link: Dev Biol

Genes referenced: LOC100887844 LOC115919910