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Echinobase
ECB-ART-31432
Dev Biol 1991 Nov 01;1481:398-401. doi: 10.1016/0012-1606(91)90348-7.
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Characterization of sea urchin egg endoplasmic reticulum in cortical preparations.

Terasaki M , Henson J , Begg D , Kaminer B , Sardet C .


Abstract
The cortical endoplasmic reticulum (ER) of sea urchin eggs was localized on isolated egg cortices by staining with aqueous suspensions of the dicarbocyanine "DiI." Immunofluorescence localization of a calsequestrin-like protein was essentially identical; this is consistent with a role for the ER in calcium regulation. The ER often encircles cortical granules, making it well-suited for initiating fusion and propagating the calcium wave. Thiazole orange and Hoechst dye 33258 at pH 2 stain ribosomes bound to the ER, providing evidence that the cortical ER is rough ER. High chloride concentrations were found to disrupt ER continuity.

PubMed ID: 1936574
Article link: Dev Biol
Grant support: [+]

Genes referenced: LOC100887844 LOC105444658