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We present a genome assembly from an individual Anseropoda placenta (Goose Foot Starfish; Echinodermata; Asteroidea; Valvatida; Asterinidae). The assembly contains two haplotypes with total lengths of 533.27 megabases and 454.27 megabases. Most of haplotype 1 (70.67%) is scaffolded into 22 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.42 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
Figure 1. . Photograph of the
Anseropoda placenta (eaAnsPlac2) specimen used for genome sequencing.
Figure 2. . Frequency distribution of
k-mers generated using GenomeScope2. The plot shows observed and modelled
k-mer spectra, providing estimates of genome size, heterozygosity, and repeat content based on unassembled sequencing reads.
Figure 3. . Hi-C contact map of the
Anseropoda placenta genome assembly.Assembled chromosomes are shown in order of size and labelled along the axes, with a megabase scale shown below. The plot was generated using PretextSnapshot.
Figure 4. . Evaluation of
k-mer completeness using MerquryFK.This plot illustrates the recovery of
k-mers from the original read data in the final assemblies. The horizontal axis represents
k-mer multiplicity, and the vertical axis shows the number of
k-mers. The black curve represents
k-mers that appear in the reads but are not assembled. The green curve corresponds to
k-mers shared by both haplotypes, and the red and blue curves show
k-mers found only in one of the haplotypes.
Figure 5. . Assembly metrics for eaAnsPlac2.hap1.1.The BlobToolKit snail plot provides an overview of assembly metrics and BUSCO gene completeness. The circumference represents the length of the whole genome sequence, and the main plot is divided into 1 000 bins around the circumference. The outermost blue tracks display the distribution of GC, AT, and N percentages across the bins. Scaffolds are arranged clockwise from longest to shortest and are depicted in dark grey. The longest scaffold is indicated by the red arc, and the deeper orange and pale orange arcs represent the N50 and N90 lengths. A light grey spiral at the centre shows the cumulative scaffold count on a logarithmic scale. A summary of complete, fragmented, duplicated, and missing BUSCO genes in the set is presented at the top right. An interactive version of this figure can be accessed on the
BlobToolKit viewer.
Figure 6. . BlobToolKit GC-coverage plot for eaAnsPlac2.hap1.1.Blob plot showing sequence coverage (vertical axis) and GC content (horizontal axis). The circles represent scaffolds, with the size proportional to scaffold length and the colour representing phylum membership. The histograms along the axes display the total length of sequences distributed across different levels of coverage and GC content. An interactive version of this figure is available on the
BlobToolKit viewer.
