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ECB-ART-54497
Mar Drugs 2025 Oct 22;2311:. doi: 10.3390/md23110411.
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Sea Cucumber (Isostichopus badionotus): Bioactivity and Wound Healing Capacity In Vitro of Small Peptide Isolates from Digests of Whole-Body Wall or Purified Collagen.

Olivera-Castillo L , Grant G , Medina-Contreras O , Cruz-López H , Carrillo-Cocom L , Cruz-Córdova A , Segura-Cadiz F , Fernández-Velasco DA , Rodríguez-Morales S , Cauich-Rodríguez JV , Moo-Puc RE , Puerto-Castillo C , Moo-Pech GJ , Uuh-Narvaez JJ , Olvera-Novoa MA , Rodriguez-Canul R .


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Low-molecular-weight peptides derived from the digestion of body wall proteins in some sea cucumber species have wound-healing and health-promoting properties, but their potency varies widely by species, growth environment, age, and season. For the first time, small peptide (1-3 kDa) fractions have been isolated from the whole-body wall of the sea cucumber Isostichopus badionotus and its constituent collagen and tested for wound healing capacity in vitro. Ultrafiltered digests (1-3 kDa) of the pure collagen, as well as 1-3 kDa digests of the whole-body wall, had potent antioxidant activities and promoted rapid wound healing in a keratinocyte scratch wound assay. Gene expression studies suggested that the wound-healing actions of the individual collagen and body wall 1-3 kDa fractions differed significantly. Low-molecular-weight peptides derived from I. badionotus collagen did promote wound healing in vitro; however, their efficacy may have been modulated by additional factors produced during body wall or collagen digestion. These findings need to be confirmed in vivo.

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